The C10orf67 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed to disrupt expression of the C10orf67 gene in HEK293T human embryonic kidney epithelial cells. This loss-of-function model enables the study of C10orf67 gene function in a widely utilized host cell line. The polyclonal knockout format maintains genetic heterogeneity, reducing clonal artifacts and providing a robust system for functional genomics and mechanistic studies.
HEK293T cells are derived from human embryonic kidney tissue and have been transformed with adenovirus 5 DNA and the SV40 large T antigen. This cell line exhibits an epithelial-like morphology and is renowned for its high transfectability and capacity for recombinant protein expression and viral vector production. The well-characterized background of HEK293T cells makes them an ideal platform for investigating the roles of extracellular matrix (ECM)-associated genes like C10orf67.
C10orf67 encodes HAPLN6, a putative hyaluronan and proteoglycan link protein that stabilizes hyaluronan-proteoglycan complexes in the ECM, thereby influencing matrix integrity and cell adhesion. Its function is regulated by upstream signals, including transforming growth factor-beta (TGF-??) through TGFBR1 and SMAD2/3, Wnt signaling via CTNNB1 and LEF1, and integrin signaling through ITGB1 and focal adhesion kinase (FAK). Downstream, C10orf67 modulates the expression and activity of matrix metalloproteinase 2 (MMP2), collagen, and fibronectin. Interacting partners include hyaluronic acid, proteoglycans, and aggrecan. Knockout of C10orf67 may impair ECM assembly and alter integrin-mediated signaling pathways.
In the HEK293T context, disruption of C10orf67 offers a valuable model to dissect ECM organization and adhesion signaling. The epithelial-like nature of HEK293T cells permits assessment of ECM component production and integrin-dependent focal adhesion dynamics, which are critical for cellular responses to mechanical cues. This polyclonal knockout pool is particularly suited for identifying phenotypic changes associated with C10orf67 loss without the confounding effects of clonal selection, making it suitable for large-scale functional screens and comparative omics analyses.
This knockout product supports a broad range of research applications, including the study of ECM dynamics, cancer cell adhesion and migration, and tumor microenvironment biology. Researchers can employ techniques such as Western blotting for collagen and fibronectin, immunofluorescence staining for focal adhesion complexes, migration and invasion assays, RNA sequencing for transcriptomic profiling, co-immunoprecipitation of hyaluronan complexes, and quantitative adhesion assays. The C10orf67 Knockout HEK293T Polyclonal Cells provide a versatile tool for functional genomics of uncharacterized genes and mechanistic investigations in signal transduction. For further details or technical support, please contact Ascent Research.
