The C16orf87 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting the C16orf87 open reading frame. This polyclonal loss-of-function model provides a versatile tool for exploring the function of this uncharacterized gene. CRISPR/Cas9-mediated gene disruption was used to generate a heterogeneous pool of cells with targeted modifications, avoiding clonal bias and enabling robust phenotypic screening.
The Jurkat cell line, derived from the peripheral blood of an acute T-cell leukemia patient, is an immortalized CD4+ T lymphocyte line widely used to study T-cell receptor signaling, HIV infection, and apoptosis. Jurkat cells respond to stimuli such as anti-CD3 antibodies and phorbol esters, making them a tractable system for dissecting signal transduction pathways. Their well-characterized biology and ease of genetic manipulation make them ideal for functional genomics studies.
C16orf87 is an uncharacterized open reading frame on chromosome 16 with no known upstream regulators, downstream targets, or interacting partners. It lacks annotated functional domains, and its role in T-cell biology remains entirely unexplored. By knocking out C16orf87 in Jurkat cells, researchers can systematically assess its potential contributions to processes like TCR-dependent activation, cytokine secretion, and cell cycle regulation. Comparative studies between wild-type and knockout polyclonal populations provide a direct avenue for deorphanizing this gene.
Utilizing the Jurkat T-cell model, this polyclonal knockout pool allows assessment of C16orf87 function within a relevant immune cell context. The polyclonal nature preserves genetic heterogeneity, facilitating detection of subtle phenotypic effects that might be missed in clonal lines. This model is particularly suited for screening assays aimed at uncovering novel regulators of T-cell signaling, apoptosis, or antiviral responses.
Researchers can employ these cells for functional characterization of C16orf87 through complementary assays, including Sanger sequencing for editing verification, RT-qPCR for transcript analysis, western blotting for protein expression, and flow cytometry for phenotyping of activation markers. Proliferation and apoptosis assays can further elucidate its role in cell fate decisions. This product supports both hypothesis-driven and discovery-based investigations, and detailed protocol information is available from Ascent Research.
