The LIAT1 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population with targeted disruption of the LIAT1 gene, providing a loss-of-function model for telomere research. These polyclonal knockout cells offer a heterogeneous edited pool, minimizing clonal artifacts, and are derived from HEK293T cells. The CRISPR-mediated disruption targets LIAT1, a telomere-binding protein central to telomere length regulation. This product serves advanced studies in telomere biology, cancer predisposition, and related disorders.
HEK293T cells, a widely used human embryonic kidney epithelial line expressing SV40 large T antigen, facilitate protein expression and lentivirus production. Their robust growth, high transfectability, and active telomerase make them an ideal host for knockout models of telomere-associated genes. The immortalized epithelial background ensures consistent telomere dynamics without replicative senescence, providing a reliable platform for LIAT1 investigation.
LIAT1 is a telomere-binding protein that interacts with ATE1 arginyltransferase to modulate telomeric arginylation, thereby regulating telomerase access and shelterin stability. This influences TERT activity and the assembly of shelterin components POT1 and TPP1. LIAT1 functions downstream of telomere dysfunction signals and the ATE1 pathway, within a network comprising TERF1, TERF2, TERT, DKC1, and TINF2, coordinating C-strand synthesis and DNA damage response. Disruption of LIAT1 leads to telomere shortening, uncapping, and DNA damage signaling, recapitulating molecular features of dyskeratosis congenita and Hoyeraal-Hreidarsson syndrome.
In the HEK293T background, LIAT1 knockout enables dissection of its role in telomere dynamics, leveraging intact shelterin and telomerase. This allows focused analysis of LIAT1-ATE1-mediated regulation of telomere extension and C-strand fill-in, free of senescence artifacts. The polyclonal format supports bulk-level assays and genetic screens, capturing dosage-sensitive phenotypic variations.
Applications include western blotting for shelterin proteins (TERF1, TERF2, POT1), qPCR-based T/S ratio telomere length measurement, and immunofluorescence for telomere dysfunction-induced foci (TIFs). Co-immunoprecipitation can evaluate LIAT1-ATE1 interactions in the knockout context. These cells are suitable for CRISPR synthetic lethality screens and drug testing targeting telomere maintenance, with relevance to cancer, aging, and inherited telomeropathies. For further information, please contact Ascent Research.
