The C19orf47 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed to disrupt the C19orf47 gene in the human Jurkat T-lymphocyte cell line. This polyclonal model maintains a heterogeneous knockout profile across the cell population, providing a robust system for studying gene function without the clonal selection biases inherent in single-cell-derived lines. The CRISPR/Cas9-mediated gene disruption enables loss-of-function analysis of C19orf47, a gene encoding a predicted transmembrane protein of unknown function.
Jurkat cells are an immortalized T-lymphocyte line originally derived from the peripheral blood of a male patient with acute T-cell leukemia. They serve as a widely accepted model for T-cell receptor (TCR) signaling and T-cell function, making them ideal for investigating components of T-cell activation, proliferation, and apoptosis. Their extensive use in immunology and cancer research provides a well-characterized background for integrating novel gene discoveries.
C19orf47 is a poorly characterized gene predicted to encode a transmembrane protein, suggesting potential roles in transport or signaling at the cell surface or within intracellular membranes. However, its molecular function, interacting partners, upstream regulators, and downstream effectors remain entirely unknown. No established biological pathways have been associated with C19orf47, and it has not been linked to known diseases. This knockout model therefore offers a valuable tool to probe its functional significance in an unbiased manner.
In the context of Jurkat T cells, disruption of C19orf47 enables researchers to examine its potential involvement in TCR-mediated signaling cascades. Given the prediction of a transmembrane topology, C19orf47 could hypothetically influence receptor-proximal events, membrane organization, or intracellular trafficking. The polyclonal knockout population allows for population-level analyses that can reveal subtle phenotypes in T-cell activation, cytokine production, or cell survival, without artifacts from clonal variation.
This product supports a wide range of research applications, including functional characterization of C19orf47, investigation of novel T-cell signaling components, and discovery of disease-associated pathways. Representative assays include Western blotting and RT-qPCR for expression analysis, RNA-seq for transcriptomic profiling, T-cell activation assays measuring IL-2 secretion or CD69 upregulation, calcium flux assays to assess early signaling events, as well as apoptosis, proliferation, and phospho-signaling analyses. For additional technical information or to place an order, please contact Ascent Research.
