The C19orf47 Knockout SK-HEP-1 Polyclonal Cells are a ready-to-use CRISPR/Cas9-edited knockout cell population designed for functional investigation of the uncharacterized protein C19orf47. This product consists of a polyclonal pool of SK-HEP-1 cells harboring a heterogeneous spectrum of CRISPR-mediated gene disruptions at the C19orf47 locus. As a polyclonal knockout model, it avoids potential biases associated with single-cell clonal selection and provides a robust loss-of-function system for studying gene function in a liver cancer context. The cells are delivered as a live culture and can be immediately expanded for diverse experimental workflows.
The host SK-HEP-1 cell line is a well-established human hepatocellular carcinoma model originally derived from the ascitic fluid of a patient with liver adenocarcinoma. This cell line retains key features of hepatic malignancy and is widely employed in cancer research to study tumor cell biology, including proliferation, migration, invasion, and drug response. Its origin from a metastatic effusion underscores its relevance for investigating advanced liver cancer mechanisms. The SK-HEP-1 background provides a relevant epithelial context for exploring the role of C19orf47 in hepatocarcinogenesis.
C19orf47 is a poorly characterized gene with no previously established biological function. The CRISPR/Cas9-mediated knockout in SK-HEP-1 cells creates a loss-of-function model that enables systematic interrogation of its potential contributions to cellular processes. Although no interacting factors or pathway affiliations have been reported, the knockout cells can be used in unbiased screening approaches to identify binding partners, transcriptional changes, and phenotypic alterations. This model serves as a foundational tool for deorphanizing C19orf47 and defining its molecular role.
Pairing SK-HEP-1 liver cancer cells with C19orf47 knockout allows researchers to investigate whether this gene modulates tumorigenic traits such as anchorage-independent growth, metabolic reprogramming, or metastatic potential. Because the SK-HEP-1 line is frequently used in xenograft tumor models, the knockout cells can be directly applied to in vivo studies to assess the impact of C19orf47 loss on tumor initiation and progression. This combination positions the product as a valuable resource for translational hepatocellular carcinoma research.
Typical applications include functional genomics, tumorigenesis assays, and gene function characterization. Researchers can perform RT-qPCR and western blotting to verify knockout at the transcript and protein levels, and employ proliferation, migration, and colony formation assays to measure phenotypic consequences. Transcriptomic analysis via RNA-seq can reveal downstream gene expression changes, while xenograft models allow assessment of in vivo tumor growth. This polyclonal knockout pool thus supports a comprehensive experimental pipeline for elucidating C19orf47 biology. For additional technical information or custom requests, please contact Ascent Research.
