C1orf174 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population targeting the C1orf174 gene. This product comprises a heterogeneous pool of HAP1 cells with targeted disruptions at the C1orf174 locus, enabling loss-of-function studies without requiring clonal isolation. The polyclonal format maintains genetic diversity while achieving efficient knockout, ideal for high-content phenotypic screens and functional genomics.
HAP1 is a near-haploid human cell line derived from chronic myeloid leukemia (CML). These fibroblast-like cells express the BCR-ABL fusion protein and maintain a predominantly haploid karyotype (with a disomic chromosome 8). Haploidy allows single-allele disruptions to yield functional knockouts, bypassing biallelic targeting. This feature, along with active CML-related signaling, positions HAP1 as an optimal system for gene function studies in a cancer-relevant framework.
C1orf174 is an uncharacterized protein-coding gene with no known molecular function, domains, or interacting partners. No upstream regulators, downstream targets, or associated pathways have been reported. The C1orf174 knockout thus offers a precise loss-of-function model to investigate its role. In the HAP1 context, disrupting C1orf174 may impact fundamental processes like proliferation, survival, and differentiation, potentially intersecting with BCR-ABL-driven oncogenic signaling.
The haploid architecture of HAP1 ensures that a single CRISPR/Cas9 gene disruption at the C1orf174 locus results in complete loss of function, without compensatory alleles. Polyclonality reduces clonal artifacts, yielding a more representative model for gene?Cphenotype studies. In the CML background, this knockout model is particularly suited to uncover roles for C1orf174 in leukemia biology, including potential effects on cell cycle regulation or drug resistance pathways.
This polyclonal knockout product is suited for functional genomics applications: high?content phenotypic screens, proliferation and colony formation assays, RNA sequencing, and Western blotting (if antibodies are available). It is a valuable resource for cancer researchers and molecular biologists investigating uncharacterized genes. Integration with HAP1 genetic toolkit enables systematic gene network dissection. For further information, contact Ascent Research.
