The C1orf198 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell pool targeting the human C1orf198 gene in the HAP1 background. This loss-of-function model enables systematic investigation of the uncharacterized C1orf198 protein, aiding functional genomics, drug target discovery, and pathway elucidation. The polyclonal format provides a heterogeneous population of edited alleles, suitable for initial phenotypic screening and validation of C1orf198-dependent cellular processes.
HAP1 is a near-haploid, adherent cell line derived from the male chronic myeloid leukemia cell line KBM-7. Its near-haploid karyotype simplifies genetic manipulation, as disruption of a single allele can yield complete loss of function. HAP1 cells retain BCR-ABL1 expression and are widely used in genetic screening, drug sensitivity profiling, and signaling studies. This host offers a robust and disease-relevant platform for examining C1orf198.
C1orf198 encodes an uncharacterized protein predicted to localize intracellularly, with no known interacting partners, regulators, effectors, or associated signaling pathways. Its molecular function and mechanistic role remain undefined, and it has not been linked to any specific disease. Disrupting C1orf198 in HAP1 cells allows unbiased functional screens to identify biological processes affected by its loss, such as cell cycle, apoptosis, migration, or signal transduction. Thus, this knockout model is a critical tool for deciphering the protein??s interactions and regulatory networks.
In the HAP1 background, C1orf198 knockout provides a clean genetic system to assess loss-of-function phenotypes without the complexity of a diploid genome. The near-haploid state ensures that targeting the single allele generates a null genotype in the polyclonal pool, enabling robust detection of functional consequences. This model is especially useful for high-throughput screens to uncover synthetic lethal interactions, pathway modulators, or compensatory mechanisms, and allows exploration of C1orf198??s potential role in leukemia biology or kinase inhibitor response.
These polyclonal knockout cells support functional genomics studies to assign biological roles to C1orf198 using assays such as Western blotting and RT-qPCR for knockout confirmation, and proliferation, migration, and apoptosis assays for phenotypic characterization. Immunofluorescence enables localization studies of reintroduced constructs. Applications include target validation, disease modeling, and pathway dissection, ultimately elucidating C1orf198??s contribution to normal and disease biology. For further details or technical support, contact Ascent Research.
