The C1orf226 Knockout HAP1 Polyclonal Cells consist of a heterogenous population of HAP1 cells harboring CRISPR/Cas9-mediated disruption of the C1orf226 gene. This polyclonal knockout pool serves as a versatile loss-of-function tool for investigating the biological role of this uncharacterized gene. Through targeted gene disruption, the cell population enables researchers to interrogate C1orf226 function across a range of phenotypic and molecular assays. The polyclonal nature of the knockout provides a representative sample of editing outcomes, facilitating robust functional studies without requiring single-cell cloning.
The HAP1 cell line is a near-haploid human cell line originally derived from a male patient with chronic myeloid leukemia. It retains the BCR-ABL1 fusion gene characteristic of the disease and displays an adherent fibroblastoid morphology. The near-haploid karyotype simplifies genetic manipulation and ensures that knockout of a single allele can result in complete loss of function for non-essential genes. This makes HAP1 an ideal host for CRISPR-based genetic screens and functional genomics studies.
C1orf226 is an uncharacterized protein-coding gene that is predicted to encode a transmembrane protein. To date, its molecular function, interacting partners, upstream regulators, and downstream targets remain unknown. Consequently, no specific signaling pathways have been associated with C1orf226. The CRISPR-mediated knockout in HAP1 cells provides a foundation for systematic phenotypic characterization, enabling the identification of biological processes in which C1orf226 participates. Through integrative approaches such as transcriptomics, interactomics, and chemogenomic profiling, the functional network of C1orf226 can be elucidated.
The C1orf226 knockout in the HAP1 background is particularly advantageous for functional annotation studies. The near-haploid genome eliminates confounding effects from a second allele, allowing clear genotype-phenotype correlations. Researchers can employ this model to dissect the protein’s role in fundamental cellular processes and disease-relevant pathways, including those pertinent to leukemia biology. The model may also facilitate synthetic lethality screens to uncover genetic vulnerabilities associated with C1orf226 loss.
The C1orf226 Knockout HAP1 Polyclonal Cells are suitable for a wide range of research applications, including functional genomics, protein function identification, synthetic lethality screening, and cancer biology. Representative assays include cell proliferation and apoptosis measurements, colony formation assays, drug sensitivity profiling, migration assays, and RNA sequencing for transcriptomic analysis. Western blotting may be performed if a validated antibody is available. This product empowers researchers to explore the uncharted biology of C1orf226. For additional information or technical support, please contact Ascent Research.
