The C1orf50 Knockout HAP1 Polyclonal Cells product is a heterogeneous population of human HAP1 cells engineered by CRISPR/Cas9-mediated disruption of the C1orf50 locus. Provided as a polyclonal knockout cell population without clonal isolation, it preserves diverse editing events. The culture is ready for expansion and loss-of-function studies.
The parental HAP1 cell line is a near-haploid human cell line derived from the KBM-7 chronic myeloid leukemia line. It exhibits an adherent fibroblast-like morphology and maintains a stable haploid karyotype in the majority of cells, which greatly facilitates the generation of knockout models. The reduced genetic redundancy inherent to the haploid state allows for unambiguous assignment of phenotypic effects to single-gene disruptions, making HAP1 an ideal host for studying uncharacterized genes such as C1orf50.
The C1orf50 gene encodes a predicted protein of unknown function, and to date no molecular interaction partners, upstream regulators, or downstream targets have been identified. Consequently, the signaling network involving C1orf50 remains completely uncharacterized. Without known activating or repressing inputs, and in the absence of validated transcriptional outputs or complex formation data, the mechanistic role of C1orf50 in cellular physiology is entirely undefined. This polyclonal knockout pool serves as a foundational reagent for pioneering investigations into the gene??s molecular functions.
By introducing loss-of-function mutations in the C1orf50 locus via CRISPR/Cas9, this knockout model enables the first direct functional interrogation of the gene in a well-characterized cellular system. The haploid background ensures that even heterozygous edits??if present in diploid subpopulations??are phenotypically exposed, while the polyclonal nature allows for the assessment of a range of disruptive alleles. This system is particularly advantageous for screening for robust phenotypes and for identifying potential genetic interactions that may be masked in diploid cells.
Researchers can utilize this polyclonal knockout cell population in a broad range of functional assays, including Western blotting, RT-qPCR, RNA-seq, cell viability and drug sensitivity testing, and flow cytometric phenotyping. The model is well-suited for systematic genetic interaction screens and synthetic lethality studies, enabling rapid linkage of C1orf50 loss to specific cellular phenotypes. For technical inquiries or ordering information, contact Ascent Research.
