The CAT Knockout A2780 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the A2780 human ovarian carcinoma cell line, targeting the CAT gene which encodes the antioxidant enzyme catalase. Supplied as a polyclonal pool, this product comprises a heterogeneous mixture of cells harboring gene disruptions induced by CRISPR/Cas9, allowing researchers to interrogate catalase-dependent phenotypes across diverse genetic backgrounds inherent to the population. This format avoids the biases of single-cell clones while maintaining the power of genetic perturbation.
The A2780 cell line is an epithelial model derived from an untreated patient with ovarian carcinoma. Established as a key tool in ovarian cancer research, A2780 cells are well-characterized for studies of tumor biology, drug response, and redox regulation. Their relevance to clinically used chemotherapeutics enhances their value for examining oxidative stress pathways in cancer progression and therapy resistance.
Catalase (CAT) is a peroxisomal heme-enzyme that decomposes hydrogen peroxide (H2O2) into water and oxygen, serving as a critical antioxidant defense. The enzyme works downstream of superoxide dismutases (SOD1, SOD2) and alongside GPX1 and peroxiredoxins to regulate intracellular ROS. CAT expression is controlled by transcription factors FOXO3a, NRF2 (NFE2L2), and PPARgamma, which respond to oxidative stress, hypoxia, and insulin/IGF-1 signaling. By eliminating H2O2, catalase protects cellular macromolecules from oxidative damage, attenuates NF-??B signaling, and inhibits apoptosis. It interacts with heme, PEX5, FoxO proteins, and p53, linking redox homeostasis to cell survival pathways.
In A2780 ovarian cancer cells, CAT knockout removes the primary H2O2 detoxification mechanism, causing ROS accumulation that may activate redox-sensitive kinases like MAPK8 (JNK) and transcription factors such as NF-??B and TP53. This disruption can impact proliferation, apoptosis, and chemoresistance, making the model highly relevant for studying oxidative stress in ovarian cancer. The polyclonal nature allows observation of diverse genetic backgrounds within the population.
These polyclonal knockout cells are suited for investigating oxidative stress responses, antioxidant defenses, and ROS-mediated signaling. Applications include catalase activity assays, H2O2 quantification, ROS detection with DCFH-DA, western blotting for oxidative markers, RT-qPCR, cell viability and apoptosis assays, metabolomic profiling, confocal imaging, and drug sensitivity testing with cisplatin or paclitaxel. This versatile model supports dissection of catalase-dependent phenotypes in ovarian cancer. For further information, contact Ascent Research.
