CAT Knockout K-562 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the K-562 human erythroleukemia line. The catalase gene (CAT) has been disrupted to create a loss-of-function model suitable for bulk analysis of redox biology and stress responses. As a polyclonal resource, this product reflects diverse editing events and avoids clonal selection artifacts, making it ideal for population-level signaling and functional studies.
The host K-562 cell line, isolated from a CML patient in blast crisis, is a BCR-ABL positive suspension line that retains the ability to differentiate along erythroid and megakaryocytic lineages. Widely used to study hematopoietic differentiation and leukemia biology, K-562 cells provide a robust platform for investigating oncogenic signaling and drug responses. Their non-adherent growth facilitates genetic manipulation and high-throughput functional assays.
Catalase, encoded by CAT, catalyzes the breakdown of hydrogen peroxide into water and oxygen, protecting cells from oxidative damage. Its transcription is regulated by FOXO3, NFE2L2 (NRF2), PPARG, and TP53, and modulated by PI3K/AKT signaling in response to ROS. Catalase interacts with PEX5 for peroxisomal localization and functions alongside SOD and GPX antioxidant systems. CAT disruption causes H?O? accumulation, activating NF-??B and AP-1 pathways, upregulating BAX and CASP3, and impacting regulators like SRC, PTEN, and p38 MAPK, thereby connecting oxidative stress to apoptosis and proliferation control.
In K-562 cells, BCR-ABL-driven oncogenic signals including PI3K/AKT intersect with redox homeostasis, making catalase knockout a valuable tool to examine how oxidative stress influences leukemia biology. Loss of catalase is expected to elevate ROS, potentially altering differentiation potential, drug sensitivity, and apoptotic thresholds. This model supports dissection of compensatory antioxidant pathways and synthetic lethal interactions specific to CML.
Key applications include studying oxidative stress responses in leukemia, evaluating drug resistance mechanisms, and exploring ROS-mediated erythroid and megakaryocytic differentiation. Representative assays encompass H2DCFDA ROS detection, catalase activity and western blot analysis, RT-qPCR for antioxidant genes, Annexin V apoptosis assays, MTT viability tests, and phospho-signaling profiling (e.g., phospho-AKT, phospho-NF-??B). These cells are also suitable for acatalasemia research and redox modulator screening. For further information or ordering, please contact Ascent Research.
