The CAT Knockout MES-OV Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population with targeted disruption of the CAT gene in MES-OV cells. This loss-of-function model enables investigation of catalase biology in human ovarian clear cell carcinoma. The polyclonal composition captures diverse genetic alterations, supporting robust functional and pharmacological studies of oxidative stress defense and redox-regulated pathways.
The MES-OV cell line originates from human ovarian clear cell carcinoma, an epithelial malignancy characterized by distinctive genetic and metabolic alterations, including dysregulation of oxidative stress responses. It retains key tumor properties, providing a highly relevant model for dissecting ovarian cancer biology, evaluating drug sensitivity, and identifying novel therapeutic targets.
Catalase (CAT) is a peroxisomal enzyme that efficiently decomposes hydrogen peroxide into water and oxygen, critically shielding cells from oxidative injury. Its expression is transcriptionally governed by NRF2 (NFE2L2), FOXO3, NF-??B, HIF1A, and PPARG, which respond to cellular redox status. Catalase activity directly modulates intracellular hydrogen peroxide levels and redox-sensitive signaling cascades involving superoxide dismutases SOD1 and SOD2, peroxiredoxin PRDX1, and glutathione peroxidase GPX1. Peroxisomal import requires interaction with PEX5, and function is integrated with the thioredoxin system (TXN, TXNRD1). Thus, CAT occupies a central position in antioxidant defense and redox signal transduction.
In MES-OV ovarian carcinoma cells, catalase neutralizes endogenous metabolic hydrogen peroxide and oxidative stress induced by chemotherapeutics. CAT knockout leads to hydrogen peroxide accumulation, redox imbalance, and potential activation of stress-responsive transcription factors. This model facilitates study of ovarian cancer cell adaptation to oxidative damage, effects on proliferation and apoptosis, and treatment resistance mechanisms. Crosstalk between catalase loss and complementary antioxidant systems, such as glutathione and thioredoxin pathways, can be systematically examined.
The polyclonal knockout pool supports diverse investigations: hydrogen peroxide signaling in tumor progression, antioxidant defense coordination, and oxidative stress-induced senescence. Standard assays include catalase immunoblotting, enzyme activity measurement, Amplex Red hydrogen peroxide quantification, and flow cytometric ROS detection with DCFH-DA. Immunofluorescence examines peroxisomal protein localization, while cell viability under oxidative challenge provides functional readouts. RT-qPCR reveals compensatory transcriptional responses. For technical assistance and ordering details, please contact Ascent Research.
