The CAT Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Raji B lymphoblastoid cell line. This product features targeted disruption of the CAT gene, which encodes catalase, an essential antioxidant enzyme. The polyclonal format provides a heterogeneous pool of cells with loss-of-function mutations in CAT, enabling robust assessment of gene function without clonal selection biases. This knockout model is designed for researchers investigating oxidative stress responses and the role of catalase in B-cell biology.
The Raji cell line is a widely used human B lymphocyte model, originally derived from a Burkitt lymphoma patient. Raji cells are EBV-positive and maintain a lymphoblastoid phenotype, making them suitable for studies in immunology and cancer biology. These suspension cells exhibit rapid proliferation and have been extensively characterized in signaling, apoptosis, and drug response assays. The host cell background provides a relevant context for examining the interplay between EBV latency and cellular antioxidant defenses.
Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen, thereby protecting cells from oxidative damage. CAT expression is transcriptionally regulated by FOXO transcription factors, NRF2, PPAR??, and HIF-1?? in response to oxidative stress. Catalase interacts with peroxisomal import receptors PEX5 and PEX14, and functionally cooperates with superoxide dismutase and glutathione peroxidase to maintain redox homeostasis. Disruption of CAT leads to accumulation of H2O2, which can modulate redox-sensitive signaling pathways and promote oxidative stress-induced apoptosis.
In the Raji cellular context, CAT knockout results in heightened sensitivity to oxidative stress, potentially impacting lymphoma cell survival and proliferation. The EBV-positive background of Raji cells may synergize with elevated reactive oxygen species (ROS), influencing viral latency and oncogenic signaling. This model enables dissection of catalase-dependent protective mechanisms in B lymphoblastoid cells, providing insights into how oxidative stress contributes to lymphomagenesis and tumor maintenance.
Researchers can employ this polyclonal knockout population to study oxidative stress responses in B cells, screen for modulators of catalase activity or ROS signaling, and investigate the role of catalase in lymphoma biology. Representative assays include catalase activity measurement, H2DCFDA-based ROS detection by flow cytometry, western blotting for catalase, cell viability assays under H2O2 challenge, Annexin V apoptosis staining, and qRT-PCR for oxidative stress markers. For further information or to inquire about this product, please contact Ascent Research.
