CAV1 Knockout AGS Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from AGS gastric adenocarcinoma cells. The product provides a heterogeneous pool of cells with targeted disruption of the CAV1 gene, enabling loss-of-function studies without clonal selection bias. This polyclonal format preserves genetic diversity while ensuring robust CAV1 deficiency across the population, ideal for high-throughput screening and functional assays.
The AGS cell line originates from a human gastric adenocarcinoma, serving as a well-characterized epithelial model for studying gastric cancer biology. AGS cells exhibit adherent morphology and retain key signaling properties of gastric epithelial cells, including responsiveness to growth factors and microbial challenges such as Helicobacter pylori infection. Their widespread use in cancer research makes them a suitable host for investigating tumorigenic mechanisms.
CAV1 encodes caveolin-1, a scaffolding protein essential for caveolae formation. Caveolin-1 regulates endocytosis, cholesterol homeostasis, and signal transduction by interacting with numerous signaling molecules. Upstream, CAV1 expression is regulated by TGF-??, cholesterol, PPAR??, FOXO transcription factors, and SREBP. Downstream, caveolin-1 interacts with and modulates the activity of eNOS, Src kinases, H-Ras, EGFR, ??-catenin, and Akt. It forms complexes with cavin family proteins, integrins, and TGF-?? receptors, integrating pathways such as caveolae-mediated endocytosis, integrin signaling, MAPK/ERK, PI3K/Akt, TGF-?? signaling, and Wnt/??-catenin signaling.
In AGS gastric adenocarcinoma cells, CAV1 knockout disrupts caveolae formation, altering integrin-mediated adhesion and signaling. Loss of caveolin-1 may relieve its tumor-suppressive interactions with growth factor receptors, potentially leading to enhanced proliferation through constitutive activation of MAPK and Akt pathways. This model is particularly relevant for dissecting the dual role of caveolin-1 in gastric cancer, where it can act as a context-dependent tumor suppressor or promoter, and for examining its contribution to processes such as epithelial-mesenchymal transition, drug resistance, and Helicobacter pylori pathogenesis.
CAV1 Knockout AGS Polyclonal Cells are a versatile tool for gastric cancer research, enabling investigations into caveolae dysfunction, endocytosis, and oncogenic signaling. Typical applications include Western blotting for CAV1 expression, immunofluorescence for caveolae assessment, cell proliferation (MTT) and invasion/migration assays, RT-qPCR for downstream targets, phospho-ERK/Akt analysis, cholesterol modulation experiments, and co-immunoprecipitation for protein interactions. These cells also support drug sensitivity profiling and H. pylori infection studies. For further information, please contact Ascent Research.
