The CAV1 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population targeting the CAV1 gene, which encodes the caveolae scaffolding protein caveolin-1. This knockout model ablates caveolin-1 expression, enabling loss-of-function studies of caveolae-dependent endocytosis, lipid regulation, and signal transduction in a human B lymphocyte background. The polyclonal format provides a heterogeneous edited population suitable for robust downstream analyses without clonal selection.
The Raji cell line, derived from a Burkitt lymphoma patient, is an Epstein-Barr virus-positive B lymphocyte model widely used in immunology and cancer research. These cells retain key B-cell functions such as antibody production and antigen presentation, and their aggressive lymphoma origin makes them an ideal host for investigating signaling pathways driving B-cell malignancies.
Caveolin-1 organizes membrane microdomains by scaffolding signaling molecules, including SRC, Ras, G-proteins, and integrins, through its binding partner PTRF/cavin-1. It is regulated by upstream factors such as SRC kinases, EGF, PDGF, TGF-??, and cholesterol, and controls downstream effectors like eNOS, MAPK, Akt, and Cyclin D1. CAV1 disruption dismantles this scaffolding network, leading to dysregulation of caveolin-mediated endocytosis, integrin signaling, and PI3K-Akt pathway activity.
In Raji cells, CAV1 knockout impairs caveolae formation and caveolar endocytosis, disrupting membrane microdomain organization and attenuating B-cell receptor and integrin signal transduction. This alteration likely impacts phosphorylation cascades involving SRC and MAPK, influencing proliferation and survival. The model provides a direct means to study how loss of caveolin-1 scaffolding modifies malignant B-cell behavior and identifies caveolin-dependent vulnerabilities in lymphoma.
This knockout cell population supports studies of caveolin-1 function in B-cell lymphomas, endocytosis trafficking, and signal transduction. Applications include phospho-signaling analysis by Western blotting, proliferation and migration assays, flow cytometry for surface markers, and immunofluorescence localization of caveolar components. It is also suitable for drug resistance profiling. For further information, please contact Ascent Research.
