The CBX3 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited population from the HEK293T cell line, designed for targeted disruption of the CBX3 gene (HP1??). This polyclonal loss-of-function model enables investigation of CBX3-dependent chromatin regulation and transcriptional silencing without clonal selection artifacts. The heterogeneous mixture of knockout alleles supports robust functional studies of heterochromatin maintenance, gene repression, and genome stability.
The HEK293T host cell line is a human embryonic kidney derivative stably expressing SV40 large T antigen, known for high transfection efficiency and strong protein expression. These cells are widely employed for transient and stable expression, viral production, and biochemical experiments. Although of kidney origin, HEK293T cells serve as a versatile platform for studying fundamental cellular processes, and their rapid growth and compatibility with CRISPR editing facilitate generation of knockout models like this CBX3-disrupted population.
CBX3 (HP1??) is a reader of histone H3 lysine 9 di- and tri-methylation (H3K9me2/3) via its chromodomain, nucleating heterochromatin assembly and transcriptional silencing. It recruits DNA methyltransferases DNMT1 and DNMT3a and histone deacetylases HDAC1/2 to lock repressive chromatin. CBX3 interacts with the H3K9 methyltransferase SUV39H1, other HP1 family members CBX1/HP1?? and CBX5/HP1??, and scaffold protein TIF1??/TRIM28. Its activity is regulated by kinases such as Aurora B and CDK1, and it transcriptionally controls adhesion gene CDH1 and cell cycle inhibitor CDKN1A/p21. Through these networks, CBX3 governs heterochromatin maintenance, DNA damage responses, and cell cycle progression.
In the HEK293T background, CBX3 knockout cells allow examination of heterochromatin dynamics and gene silencing in a highly transfectable context. The polyclonal nature avoids clonal bias while assessing global effects on H3K9me3 distribution and transcriptional reprogramming. Given CBX3??s involvement in breast, ovarian, and lung cancers, these cells provide a relevant model for cancer epigenetic studies and therapeutic target validation.
This product supports a range of assays including western blotting for CBX3 and H3K9me3, RT-qPCR and RNA-seq for transcriptomic profiling, ChIP-qPCR for chromatin occupancy, immunofluorescence for localization, and co-immunoprecipitation for protein interactions. Functional assays such as flow cytometric cell cycle analysis, colony formation, and drug sensitivity testing are also applicable. These cells are ideal for chromatin biology, epigenetic regulation, and cancer research. For more information, contact Ascent Research.
