The CBX3 Knockout HT29 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the HT29 colorectal adenocarcinoma cell line. This product enables loss-of-function studies of CBX3 (HP1??), a heterochromatin protein 1 gamma. The polyclonal pool contains a diverse array of CBX3-disrupted cells, providing a robust model for investigating gene function without clonal artifacts. CRISPR/Cas9-mediated gene disruption targets CBX3, allowing exploration of its roles in epigenetic regulation, cell cycle control, and senescence in colorectal cancer.
HT29 cells, established from a primary colorectal adenocarcinoma of a 44-year-old female, serve as a well-characterized intestinal epithelial model. These adherent cells retain key colorectal cancer features, including aberrant Wnt/??-catenin signaling, and can form polarized monolayers. The CBX3 knockout in HT29 cells provides a disease-relevant system to study chromatin-mediated regulatory mechanisms in colorectal cancer biology.
CBX3 binds H3K9me2/me3 via its chromodomain, facilitating heterochromatin formation and transcriptional repression. It recruits SUV39H1 to propagate H3K9me3, silencing tumor suppressors like CDKN1A (p21) and CDKN2A (p16). Upstream regulators include E2F transcription factors and p53, while downstream effectors involve the RB1 pathway. CBX3 interacts with HP1 family members (CBX1, CBX5), TRIM28, and CHAF1A. In colorectal cancer, CBX3 overexpression promotes proliferation and senescence bypass, making its disruption a valuable tool to study these processes.
This CBX3 knockout model enables dissection of heterochromatin-mediated gene silencing in colorectal cancer. The polyclonal nature avoids clonal artifacts, offering a representative population for functional studies. Researchers can investigate cross-talk with Wnt/??-catenin and p53 pathways, and assess effects on chromatin landscape, gene expression, and cellular phenotypes such as proliferation and migration.
Typical applications include cell proliferation (MTT, colony formation), migration assays, senescence-associated ??-galactosidase staining, ChIP-qPCR for H3K9me3, RT-qPCR, RNA-seq, and immunofluorescence. This product is suited for epigenetic drug sensitivity screens and studies of CBX3-dependent gene regulation. For further information, please contact Ascent Research.
