The CBX8 Knockout AGS Polyclonal Cells consist of a CRISPR/Cas9-edited polyclonal knockout cell population derived from the AGS human gastric adenocarcinoma cell line. This product provides a heterogeneous loss-of-function model for studying CBX8-dependent functions, avoiding the clonal selection biases that can arise from single-cell-derived knockout lines.
The AGS cell line is an epithelial cell line isolated from a patient with gastric adenocarcinoma. It is a well-established in vitro model for gastric cancer research, retaining key features such as deregulated proliferation, resistance to apoptosis, and the capacity to form tumors in xenograft models. These characteristics make AGS cells ideal for investigating tumor cell biology, signaling pathways, and therapeutic responses.
CBX8 is a chromodomain-containing protein that serves as a core component of Polycomb Repressive Complex 1 (PRC1). It directly recognizes trimethylated lysine 27 on histone H3 (H3K27me3), a mark deposited by PRC2, and facilitates chromatin compaction to maintain stable gene silencing. CBX8 is regulated by the transcription factors E2F1 and c-Myc and interacts with PRC1 subunits including PHC1, PHC2, RING1A, and RING1B, as well as the PRC2 catalytic subunit EZH2. Downstream, CBX8 represses key tumor suppressors such as CDKN2A (p16/INK4a) and CDKN2B (p15), along with HOX genes and Wnt target genes. In gastric cancer, CBX8 overexpression promotes tumorigenesis by silencing these loci and activating stem cell pathways through Wnt/??-catenin and PI3K/AKT signaling.
The CBX8 Knockout AGS Polyclonal Cells offer a powerful tool to dissect the contribution of CBX8 to gastric cancer pathogenesis. By disrupting CBX8 in the AGS background, researchers can examine its role in PRC1-mediated gene silencing, H3K27me3 maintenance, and the regulation of proliferation and stemness. This model is particularly relevant for studies on tumor initiation and drug resistance, as CBX8 loss may reactivate tumor suppressors and impair stem cell properties. Moreover, the polyclonal knockout population captures a range of editing outcomes, providing a comprehensive view of CBX8 function.
This product supports a variety of applications in epigenetic research, including ChIP-qPCR for H3K27me3, RNA-seq transcriptomic profiling, RT-qPCR, and western blotting. Functional assays such as sphere formation, flow cytometry for cell cycle and apoptosis, migration/invasion, and drug sensitivity testing with cisplatin can be employed to characterize CBX8-dependent phenotypes. Researchers studying PRC1 biology, gastric cancer epigenetics, or therapeutic resistance mechanisms will find this knockout model highly useful. For additional information, please contact Ascent Research.
