The CC2D1A Knockout HeLa Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from HeLa cells, with targeted disruption of the CC2D1A gene. This heterogeneous pool provides a robust loss-of-function model without clonal selection, enabling studies of CC2D1A??s role in signal transduction. By eliminating functional CC2D1A protein, the knockout facilitates dissection of its scaffolding functions in pathways central to inflammation and apoptosis.
HeLa cells, an immortalized cervical adenocarcinoma line established in 1951 from Henrietta Lacks?? biopsy, are a mainstay of biomedical research. These epithelial cells exhibit rapid proliferation and have been pivotal in cancer and signaling studies. Their HPV18-positive background makes them relevant for investigating NF-??B pathway dysregulation. Using HeLa as the host for CC2D1A knockout allows characterization of gene function in a well-defined cancer cell context.
CC2D1A is a scaffold protein essential for TNF-??-mediated NF-??B activation. Upon TNF-?? binding, CC2D1A assembles at the TNFR1 complex, interacting with TRAF2, RIP1, and the IKK complex (IKK-??/??, NEMO). This promotes IKK-dependent phosphorylation and degradation of I??B, enabling NF-??B nuclear translocation and transcriptional induction of target genes like IL-6, IL-8, Bcl-xL, and cIAP1/2. Thus, CC2D1A bridges receptor-proximal events to downstream transcriptional programs regulating inflammation and cell survival.
In HeLa cells, where constitutive NF-??B activity supports oncogenic phenotypes, CC2D1A knockout impairs maximal pathway activation in response to stimuli such as TNF-?? and IL-1??. This model allows precise investigation of CC2D1A??s contribution to cytokine-induced gene expression and apoptotic regulation under stress. Comparative studies between wild-type and knockout populations can identify CC2D1A-dependent signaling nodes, offering insights into therapeutic targets in NF-??B-driven cancers and inflammatory disorders.
This CC2D1A knockout is suited for diverse applications: NF-??B signaling studies, inflammation research, apoptosis mechanisms, and cancer cell biology. Key assays include Western blotting for pathway phospho-proteins, RT-qPCR for target gene expression, luciferase reporters for NF-??B activity, co-immunoprecipitation for complex assembly, and apoptosis assays (caspase-3/7, Annexin V). The model also supports neurodevelopmental research, given CC2D1A??s link to intellectual disability and autism. For additional details or orders, please contact Ascent Research.
