The CCDC102A Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed to disrupt the CCDC102A gene in the HEK293T human cell line. This product provides a loss-of-function model for investigating the biological role of CCDC102A, a poorly characterized coiled-coil domain-containing protein. The polyclonal pool ensures a range of genetic disruptions across the population, enabling robust phenotypic analysis and minimizing clonal artifacts.
HEK293T cells are a derivative of the HEK293 human embryonic kidney epithelial cell line, stably transformed with the SV40 large T-antigen to enhance episomal replication and protein production. Their adherent epithelial morphology and high transfectability have established HEK293T as a premier host for recombinant protein expression, lentivirus production, and biochemical studies. This cellular background offers a relevant and experimentally tractable context for dissecting CCDC102A function in human epithelial biology.
CCDC102A is annotated as a coiled-coil domain-containing protein, a structural motif commonly associated with protein?Cprotein interaction scaffolds. While its precise molecular function remains undefined, domain predictions suggest potential involvement in cytoskeletal organization or ciliary assembly processes. At present, no upstream regulators, downstream effectors, or direct interaction partners have been reported for CCDC102A, highlighting the unmet need for functional characterization that this knockout model addresses.
Within the HEK293T epithelial setting, CCDC102A disruption may reveal insights into processes such as actin cytoskeleton remodeling, microtubule dynamics, or primary cilium formation. The host cell’s extensively documented signaling networks and amenability to imaging and biochemical fractionation make it possible to probe CCDC102A localization and complex formation with high resolution. This model thus serves as a valuable platform for hypothesis-driven research into the scaffolding roles of uncharacterized coiled-coil proteins.
This polyclonal knockout population is suitable for a wide array of experimental approaches, including co-immunoprecipitation to discover novel protein interactions, immunofluorescence to map subcellular distribution, and western blotting or RT-qPCR to confirm gene disruption. Researchers can employ these cells in phenotypic screens assessing cell morphology, migration, or proliferation. By enabling the first functional dissection of CCDC102A, these knockout cells fill a critical gap in the study of coiled-coil protein biology. For additional information or to place an order, please contact Ascent Research.
