The CCDC102A Knockout HT29 Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population derived from the HT29 human colorectal adenocarcinoma cell line. This loss-of-function model is generated through CRISPR/Cas9-mediated disruption of the target gene, producing a heterogeneous cell pool with targeted CCDC102A knockout. The polyclonal format offers a practical tool for studying the consequences of gene disruption while maintaining cellular heterogeneity, suitable for pooled assays and cost-effective screening.
HT29 is an established epithelial cell line originally isolated from a primary human colorectal adenocarcinoma. It retains key characteristics of intestinal epithelium, including differentiation capacity under defined conditions, and is widely used as a colorectal cancer model. The line exhibits stable growth and has been characterized for tumorigenesis, cell adhesion, and drug response studies, making it an appropriate host for knockout studies of colorectal cancer-associated genes.
CCDC102A encodes a coiled-coil domain-containing protein predicted to function in cytoskeletal organization and cell adhesion. While its direct regulators, downstream effectors, and interacting partners remain unknown, the presence of a coiled-coil domain suggests participation in protein?Cprotein interactions critical for actin dynamics and cell-matrix adhesion. Coiled-coil domains often mediate assembly with scaffolding proteins and cytoskeletal linkers, implying CCDC102A may integrate into networks coordinating cell adhesion and migration. This knockout model is valuable for revealing its mechanistic roles.
Disrupting CCDC102A in HT29 cells is expected to impair cytoskeletal architecture and adhesion, providing a relevant model to study its contribution to colorectal cancer progression. As cell adhesion and migration are central to tumor invasion and metastasis, this knockout can be used to examine alterations in epithelial morphology, integrin signaling, and cell junction integrity. The HT29 background ensures findings are contextualized within colorectal cancer biology, aiding translational research.
Applications include functional characterization by Western blotting and immunofluorescence for cytoskeletal markers (e.g., actin, vinculin), quantitative cell adhesion and migration/invasion assays, proliferation assays, and transcriptomic analysis via RNA-seq. Researchers can compare knockout and wild-type HT29 cells to validate CCDC102A as a therapeutic target or investigate its role in tumorigenesis. For further information, please contact Ascent Research.
