The CCDC117 knockout A2780 polyclonal cells are a CRISPR/Cas9-edited population designed for loss-of-function analysis of the CCDC117 gene in a human ovarian carcinoma cell background. The polyclonal knockout cells harbor a heterogeneous array of CCDC117-disrupted alleles, avoiding clonal biases while maintaining effective gene disruption. This product offers a ready-to-use model for functional studies without the need for single-cell cloning.
The A2780 cell line is an epithelial ovarian carcinoma model originally isolated from an untreated patient with ovarian adenocarcinoma. These cells exhibit an adherent epithelial morphology and are widely employed in ovarian cancer research due to their well-characterized cisplatin-sensitive phenotype. A2780 cells retain functional DNA damage response and apoptotic pathways, making them a standard system for studying platinum-based chemotherapy response and resistance mechanisms.
CCDC117 encodes a centriolar satellite protein required for primary cilium assembly. It interacts with PCM1, CEP290, and OFD1 at the pericentriolar matrix and functions upstream of the ciliary trafficking protein IFT88 and ARL13B. CCDC117 thereby modulates Hedgehog signaling, influencing the GLI transcription factors GLI1 and GLI2. Transcription of CCDC117 is regulated by RFX factors and FOXJ1. Disruption of CCDC117 impairs centriolar satellite integrity and downstream ciliary signaling.
In the A2780 ovarian cancer context, knockout of CCDC117 is expected to impair primary cilium formation, thereby perturbing Hedgehog signal transduction and cell cycle regulation. Loss of cilium-dependent signaling can influence proliferation and cisplatin sensitivity, providing a model to dissect ciliary contributions to ovarian tumor biology. The polyclonal nature of the knockout population mirrors the heterogeneity of tumor cell populations, making it particularly relevant for studying dynamic cellular responses.
These polyclonal knockout cells support applications such as cilia immunostaining with ARL13B or ??-tubulin, western blotting, and RT-qPCR for Hedgehog targets like GLI1 and GLI2. Functional assays include proliferation, cell cycle flow cytometry, and cisplatin sensitivity studies, enabling dissection of ciliary and oncogenic signaling crosstalk. The model also facilitates examination of upstream regulators FOXJ1 and RFX transcription factors. For additional information, please contact Ascent Research.
