CCDC12 Knockout HeLa Polyclonal Cells consist of a heterogeneous population of HeLa cells in which the CCDC12 gene has been disrupted by CRISPR/Cas9-mediated gene editing. This polyclonal knockout product provides a robust loss-of-function model for investigating the biological function of the poorly characterized coiled-coil domain protein CCDC12. Unlike clonal isolates, the polyclonal format maintains genetic diversity, enabling broad assessment of knockout effects while minimizing clonal artifacts. This cell population serves as a ready-to-use tool for functional genomics and cell biology studies.
The HeLa cell line is derived from a HPV18-positive cervical adenocarcinoma and represents a classic model of epithelial cancer. Constitutive expression of the HPV18 E6 and E7 oncoproteins leads to inactivation of the tumor suppressor proteins p53 and Rb, respectively, resulting in a highly proliferative and genomically unstable phenotype. These features make HeLa cells a standard platform for studying cancer-related pathways, drug responses, and cytoskeletal dynamics.
CCDC12 is a coiled-coil domain-containing protein with no established disease associations and largely unknown molecular function. Based on domain architecture, it is predicted to engage in protein?Cprotein interactions typical of coiled-coil proteins, possibly contributing to ciliary assembly or cytoskeletal organization. No upstream regulators, downstream effectors, or interaction partners have been conclusively identified; however, its putative involvement in ciliogenesis and cytoskeletal regulation makes it an intriguing target for exploratory research.
In the HeLa background, which retains the basal body and molecular components required for primary cilium formation, CCDC12 knockout allows investigation of its role in ciliogenesis under serum starvation-induced conditions. Moreover, because cytoskeletal rearrangements are integral to cancer cell migration and proliferation, this knockout model may uncover novel links between CCDC12 and actin or microtubule dynamics in a p53/Rb-deficient setting.
Typical applications include Western blotting and RT-qPCR for knockout validation, immunofluorescence to monitor CCDC12 localization or ciliary markers, and functional assays such as proliferation, migration, and invasion tests. RNA-seq can be employed to globally profile transcriptomic changes upon CCDC12 loss. This product is suitable for functional genomics, cancer biology, and cilia research. For technical assistance or ordering information, please contact Ascent Research.
