The CCDC124 Knockout HEK293T Polyclonal Cells product comprises a CRISPR/Cas9-edited polyclonal knockout cell population targeting the Homo sapiens CCDC124 (GNL3L) gene. This loss-of-function model is generated using CRISPR/Cas9-mediated gene disruption, producing a genetically heterogeneous pool of HEK293T cells with abrogated CCDC124 expression. The polyclonal format obviates clonal selection artifacts while providing a robust system for studying gene function in cellular processes such as ribosomal biogenesis and proliferation control.
The parental cell line, HEK293T, is a human embryonic kidney epithelial line stably expressing the SV40 large T-antigen. This feature promotes episomal amplification of plasmids containing the SV40 origin of replication, leading to high-level transient protein expression and efficient production of recombinant viral vectors. HEK293T cells are favored for their ease of culture, rapid proliferation, and suitability for biochemical and cell biological assays.
CCDC124, also known as GNL3L, is a nucleolar GTP-binding protein essential for ribosome maturation and growth control. It functions downstream of the oncogenic transcription factor MYC and the mTORC1 kinase complex, which are master regulators of protein synthesis and metabolism. CCDC124 physically interacts with key nucleolar proteins, including Nucleostemin (GNL3) and Nucleophosmin (NPM1), to coordinate rRNA processing and ribosomal subunit assembly. Consequently, its disruption impairs ribosomal biogenesis and attenuates cell proliferation, linking nucleolar dynamics to cell cycle progression.
In the HEK293T cellular context, CCDC124 knockout induces nucleolar stress and compromises translational capacity. Given the high intrinsic proliferative and biosynthetic activity of HEK293T cells, loss of CCDC124 sensitizes cells to ribosomal biogenesis defects, potentially triggering p53-dependent or independent cell cycle arrest. This polyclonal knockout population models heterogeneous cellular responses to CCDC124 ablation, enabling dissection of mTOR?Cribosome cross-talk and nucleolar stress pathways.
This product is applicable to a range of research areas, including ribosomal biogenesis, cancer cell proliferation mechanisms, and drug target validation. Typical assays include rRNA processing analysis by RT-qPCR, cell proliferation measurements via flow cytometry, nucleolar morphology assessment by immunofluorescence, and protein expression profiling by Western blotting. For further technical information or support, please contact Ascent Research.
