The CCDC127 Knockout HEK293T Polyclonal Cells product consists of a polyclonal population of HEK293T cells engineered via CRISPR/Cas9-mediated disruption of the human CCDC127 gene. This pooled format generates a loss-of-function model in which a heterogeneous collection of gene-edited alleles enables robust assessment of CCDC127 function without the biases inherent to single-clone expansion. The polyclonal design preserves biological variability while providing a reliable platform for downstream functional studies. Researchers can utilize this knockout model to interrogate the role of CCDC127 in cellular processes ranging from protein interaction network dynamics to potential cytoskeletal or ciliary functions.
The parental HEK293T cell line is derived from human embryonic kidney epithelial cells and stably expresses the SV40 large T antigen. This modification confers high transfection efficiency and episomal replication of plasmids containing the SV40 origin of replication, making HEK293T a widely adopted host for recombinant protein expression, lentivirus and retrovirus production, and gene editing applications. The cells exhibit adherent, epithelial morphology and are grown under standard mammalian cell culture conditions, ensuring compatibility with a broad array of experimental workflows.
CCDC127 encodes a coiled-coil domain-containing protein whose molecular function remains poorly characterized. Coiled-coil domains typically mediate protein-protein interactions, suggesting that CCDC127 may serve as a scaffold to organize multiprotein complexes. Although no specific upstream regulators, downstream targets, or interacting partners have been definitively identified for CCDC127, the knockout in HEK293T cells provides a clean genetic background to systematically discover these elements through biochemical and proteomic approaches. The absence of prior knowledge underscores the value of this model for pioneering investigations into CCDC127 biology.
The integration of the CCDC127 knockout into the HEK293T background combines a physiologically relevant epithelial cell type with an experimentally tractable system. Given the high transfectability of HEK293T cells, researchers can efficiently perform complementation studies, express tagged CCDC127 constructs, and apply proximity-labeling techniques to map the CCDC127 interactome. This model is particularly suited for studying potential roles of CCDC127 in cellular structures such as the cytoskeleton or primary cilium, where coiled-coil proteins often contribute to molecular scaffolding and transport.
Typical applications of these polyclonal knockout cells include functional characterization of CCDC127 through loss-of-function phenotypes assessed via proliferation, viability, and migration assays. The identification of interaction partners can be accomplished using co-immunoprecipitation coupled with mass spectrometry, while subcellular localization studies can employ immunofluorescence microscopy. Transcriptomic analysis by RNA-seq allows exploration of downstream gene expression changes resulting from CCDC127 disruption. These cells also serve as a comparator for phenotypic rescue experiments. For further details or technical assistance, please contact Ascent Research.
