The CCDC136 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-mediated gene-disrupted cell population for studying CCDC136 function. These polyclonal knockout cells are generated in the human near-haploid HAP1 cell line, offering a stable background for loss-of-function analyses. The polyclonal pool captures heterogeneous edited alleles, enabling robust assessment without clonal artifacts. This tool facilitates investigation of CCDC136 roles in cytoskeletal regulation and acrosome biogenesis.
HAP1 is a chronic myeloid leukemia (CML)-derived near-haploid human cell line widely used in haploid genetic screens because its single-copy genome simplifies gene-editing and phenotypic analysis. Its near-haploid karyotype reduces genetic redundancy, allowing direct genotype-phenotype correlations. HAP1 cells are favored for functional genomics, CRISPR screens, and drug-target validation due to their human origin, rapid growth, ease of manipulation, and high-throughput compatibility. This background makes them ideal for CCDC136 knockout studies to dissect gene function in a controlled context.
CCDC136 encodes a coiled-coil domain protein critical for acrosome formation during spermatogenesis, potentially regulating cytoskeletal dynamics. It interacts with coiled-coil domain proteins, acrosomal matrix proteins, actin, and tubulin to mediate vesicle fusion and acrosome assembly. Regulation occurs via spermatogenic transcription factors such as CREM and SOX family members, downstream of FSH receptor/cAMP/CREB signaling. CCDC136 deficiency impairs acrosome biogenesis and sperm-egg fusion, leading to male infertility. Key partners include SPACA1, ZPBP, acrosin, and zona pellucida binding proteins.
Although HAP1 cells do not undergo spermatogenesis, the CCDC136 knockout offers a model to study its fundamental roles in cytoskeletal organization and membrane trafficking. The near-haploid genome eliminates redundancy, enabling clear dissection of CCDC136 interactions and regulatory networks. This model can reveal conserved cellular functions underlying acrosome biogenesis and provides insights into male infertility mechanisms.
Applications include functional genomics of male infertility, acrosome biogenesis studies, and gene-editing validation. Assays such as western blotting, RT-qPCR, RNA-seq, immunofluorescence, and co-immunoprecipitation are compatible. These cells enable haploid genetic screens for synthetic lethality or chemical sensitivities related to spermatogenic failure. For technical support or ordering, contact Ascent Research.
