The CCDC14 Knockout A2780 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population with disruption of the CCDC14 gene in the human ovarian carcinoma cell line A2780. This polyclonal mix comprises cells harboring varied genetic edits, offering a robust loss-of-function system while minimizing clonal bias. The use of CRISPR/Cas9 technology ensures efficient target-gene ablation, making this product ideal for functional genomics studies in ovarian cancer research.
The A2780 cell line, derived from an untreated ovarian adenocarcinoma patient, is a widely accepted model for ovarian cancer studies. It displays characteristic features such as rapid proliferation and chemosensitivity, and is used extensively to investigate tumor biology, signaling networks, and drug resistance. Its well-characterized genome and experimental tractability provide a relevant cellular context for gene knockout experiments.
CCDC14 encodes a coiled-coil domain-containing protein that localizes to the centrosome and basal body, where it is believed to participate in centriole duplication and ciliation. The protein is implicated in the centrosome cycle and cell cycle regulation, potentially acting during the initial stages of procentriole formation. CCDC14 is thought to function within the network of core centriole biogenesis factors, which includes Plk4, SAS-6, STIL, and CPAP, although its exact molecular interactions remain undefined. Disruption of CCDC14 may perturb the ordered assembly of centriolar components, leading to centrosome amplification, mitotic defects, and compromised primary cilium formation. This knockout model is therefore a valuable asset for dissecting the mechanistic contributions of CCDC14 to centrosome homeostasis and ciliary signaling.
In A2780 ovarian carcinoma cells, CCDC14 knockout enables investigation of how centrosome and ciliary defects impact cancer cell behavior. Aberrant centrosome numbers and dysregulated ciliogenesis are common in ovarian cancer, contributing to chromosomal instability and malignant progression. This model facilitates the examination of CCDC14’s role in maintaining centrosome integrity, cell proliferation, migration, and response to chemotherapeutic agents, providing insights into novel oncogenic mechanisms.
Typical applications include Western blotting, RT-qPCR, and immunofluorescence to confirm knockout and assess centrosome/cilia morphology. Cell cycle analysis by flow cytometry, proliferation assays, migration assays, and drug sensitivity screens can be performed using this polyclonal population. The CCDC14 Knockout A2780 Polyclonal Cells thus serve as a versatile tool for functional studies in centrosome biology, ciliogenesis, and ovarian cancer. For technical inquiries and ordering, please contact Ascent Research.
