The CCDC149 Knockout HEK293T Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal cell population with disrupted CCDC149 gene function. This pool of HEK293T cells carries heterogeneous edits at the target locus, enabling loss-of-function studies without clonal selection. The polyclonal format provides a robust model to investigate CCDC149 biology while minimizing clonal artifacts.
HEK293T cells are human embryonic kidney epithelial cells expressing SV40 large T antigen, offering high transfection efficiency and protein expression. They are widely used in molecular and cellular biology for genetic manipulation, signal transduction studies, and drug screening. Their rapid growth and epithelial origin make them suitable for studying intracellular trafficking and cytoskeletal organization.
CCDC149 encodes a coiled-coil domain-containing protein, a motif class that mediates protein-protein interactions. Its exact molecular function is uncharacterized, but it likely participates in assembling multiprotein complexes or regulating intracellular trafficking. Potential interacting partners include coiled-coil domain proteins, actin-associated factors, or microtubule-binding proteins, implying roles in cytoskeletal dynamics or ciliary processes. Disruption of CCDC149 in HEK293T cells abolishes these putative interactions, creating a model to identify its binding partners and downstream effects through proteomic and functional assays.
In the HEK293T background, CCDC149 knockout may affect epithelial cell morphology, adhesion, or signaling due to its possible cytoskeletal associations. The polyclonal knockout approach captures population-level effects, suitable for unbiased phenotypic screens. Researchers can leverage HEK293T??s transfectability to perform rescue experiments, validating CCDC149??s role in pathways involving coiled-coil proteins. This model offers a discovery tool for exploring links to ciliopathies or cancer.
Applications include confirming knockout via Western blotting and RT-qPCR, assessing subcellular localization by immunofluorescence, and identifying interaction partners through co-immunoprecipitation. Transcriptomic analysis with RNA-seq can reveal CCDC149-dependent gene networks. The cells are suitable for functional characterization studies, protein interaction mapping, and disease-relevant phenotypic assays. For further information, please contact Ascent Research.
