The CCDC149 Knockout HeLa Polyclonal Cells product provides a heterogeneous population of HeLa cells with CRISPR/Cas9-mediated disruption of the CCDC149 gene, creating a polyclonal loss-of-function model. This pooled knockout format preserves genetic diversity while ensuring target gene disruption, enabling functional studies without clonal selection artifacts. The polyclonal nature offers experimental robustness, making it suitable for unbiased characterization of CCDC149 in a well-established human epithelial cell background.
The host HeLa cell line is an immortalized human cervical adenocarcinoma line (Homo sapiens, female) positive for human papillomavirus type 18 (HPV18). The viral oncoproteins E6 and E7 inactivate the tumor suppressors p53 and Rb, respectively, promoting uncontrolled proliferation and providing a widely used model for cancer biology. This genetic context makes HeLa cells particularly relevant for investigating genes potentially involved in oncogenic processes, as well as for general cell biological studies of epithelial cancers.
CCDC149 is predicted to encode a coiled-coil domain-containing protein, a structural motif known to mediate protein?Cprotein interactions, suggesting CCDC149 may function as a scaffold or adapter. While its precise molecular partners and signaling networks remain uncharacterized, the mechanistic summary indicates potential roles in cytoskeletal organization or intracellular trafficking. Coiled-coil proteins often participate in diverse complexes, such as those governing vesicle transport or ciliogenesis, hinting that CCDC149 disruption could impact these fundamental processes in HeLa cells.
In the HeLa cervical adenocarcinoma context, where HPV-mediated transformation disrupts key regulatory pathways, CCDC149’s contribution to cellular architecture and dynamics may be particularly revealing. Although upstream regulators and downstream effectors are undetermined, this knockout model enables systematic interrogation of CCDC149??s influence on processes like cell proliferation, migration, and invasion??phenotypes critical to cancer progression. The lack of functional annotation underscores the value of this tool for de novo discovery.
Researchers can employ this CCDC149 knockout polyclonal population in a range of assays to elucidate gene function. Knockout validation through western blotting and RT-qPCR confirms disruption, while immunofluorescence microscopy can visualize its impact on cellular structures. Functional studies might include cell proliferation, migration, and invasion assays to assess oncogenic contributions, and co-immunoprecipitation plus mass spectrometry to identify interacting partners. Global transcriptomic changes can be profiled via RNA-seq, offering insights into CCDC149??s role. For detailed product inquiries or technical support, please contact Ascent Research.
