The CCDC152 Knockout HEK293T Polyclonal Cells are a pooled population of HEK293T cells engineered using CRISPR/Cas9 to disrupt the CCDC152 gene, creating a versatile loss-of-function model. This polyclonal format provides a heterogeneous ensemble of edited cells suitable for studying gene function in a transient, population-level context. The polyclonal nature allows rapid generation and broad applicability in functional genomics without the need for single-cell cloning.
The parental HEK293T cell line is a human embryonic kidney epithelial cell line widely utilized for high-efficiency transfection and recombinant protein expression. These cells stably express the SV40 large T antigen, enabling episomal replication of plasmids containing the SV40 origin of replication, which significantly enhances transient protein expression. The well-characterized growth properties, molecular toolbox availability, and robust transfection efficiency make HEK293T cells a preferred host for gene editing and functional studies.
CCDC152 encodes a protein containing a coiled-coil domain, a structural motif known to mediate protein?Cprotein interactions, often through homodimerization or heterodimerization. Though the precise biological role remains poorly characterized, the coiled-coil architecture suggests potential involvement in intracellular scaffolding, vesicular trafficking, or cytoskeletal organization. Current knowledge indicates that upstream regulatory inputs and downstream effectors are unknown, but the protein is predicted to associate with microtubule-related pathways. Its coiled-coil domain may facilitate interactions with other coiled-coil proteins, possibly influencing microtubule stability or motor protein complexes. Further elucidation of CCDC152??s binding partners will be critical to understanding its function.
Knocking out CCDC152 in the HEK293T background provides a clean system to dissect its cellular functions. The HEK293T line??s epithelial origin and ease of genetic manipulation permit investigations into potential roles in intracellular organization. Given that coiled-coil domain proteins are frequently implicated in ciliopathies and cytoskeletal disorders, this knockout model may serve as a tool to explore candidate links to such conditions. The absence of known disease associations makes this a discovery-driven research product, ideal for laboratories aiming to uncover novel gene functions.
Typical applications include functional characterization through transcriptomic profiling (RNA-seq), confirmation of knockout via RT-qPCR and Western blotting, and localization studies by immunofluorescence. Co-immunoprecipitation can be employed to identify CCDC152 protein interactors, while phenotypic assays may assess effects on microtubule dynamics or vesicular transport. The polyclonal population is well-suited for high-throughput screening approaches. For inquiries regarding protocol customization or specialized applications, please contact Ascent Research.
