The CCDC167 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed for targeted disruption of the CCDC167 gene in human HEK293T cells. This loss-of-function model facilitates investigation of CCDC167 function in mitotic spindle assembly and microtubule dynamics. The polyclonal format provides a heterogeneous genetically edited pool, ideal for pooled phenotypic screening and functional assays where clonal homogeneity is not essential.
HEK293T is a human embryonic kidney epithelial cell line transformed with adenovirus type 5 DNA and stably expressing the SV40 large T antigen. This genetic background permits episomal replication of plasmids containing the SV40 origin, leading to high-level recombinant protein expression and efficient lentiviral/retroviral particle production. The cells are highly transfectable and widely employed as a versatile platform for studying protein function, signal transduction, and viral packaging, offering an optimal host for gene-edited models.
CCDC167 encodes a coiled-coil domain-containing protein with a proposed role in mitotic progression and microtubule organization. It may be transcriptionally regulated by E2F factors and functionally interact with microtubule-associated proteins (MAPs) to modulate spindle architecture. CCDC167 is capable of self-dimerization and is linked to a mitotic regulatory network that includes Aurora kinase A, polo-like kinase 1 (PLK1), BUBR1, CDC20, and TPX2. CRISPR/Cas9-mediated disruption of CCDC167 is expected to compromise spindle assembly and chromosome segregation, likely resulting in mitotic arrest and cytokinesis failure.
Utilizing HEK293T as the host cell line offers distinct advantages for studying CCDC167 knockout phenotypes. The rapid proliferation rate and ease of cell cycle synchronization enable precise manipulation and analysis of mitotic stages. Furthermore, the robust protein expression machinery of HEK293T cells supports rescue experiments and structure-function analyses of CCDC167 domains. Given the putative oncogenic function of CCDC167, this model serves as a valuable tool for cancer biology research, including target validation and assessment of mitotic checkpoint vulnerabilities.
This polyclonal knockout cell product is amenable to diverse experimental workflows. Users can perform Western blotting to verify CCDC167 ablation and detect mitotic markers, RT-qPCR for transcript quantification, and immunofluorescence with anti-??-tubulin to visualize spindle morphology. Flow cytometry enables cell cycle distribution and Annexin V apoptosis analyses, while proliferation assays gauge growth defects. Co-immunoprecipitation can be employed to examine protein interactions with CCDC167. Such assays position this model for mechanistic studies in cell cycle regulation, cancer cell biology, and drug target identification. For product inquiries or technical support, please contact Ascent Research.
