CCDC167 Knockout NCI-H1299 Polyclonal Cells are a CRISPR/Cas9-edited, non-clonal knockout cell population designed to disrupt expression of the coiled-coil domain-containing protein CCDC167 in the human non-small cell lung cancer (NSCLC) line NCI-H1299. Supplied as a polyclonal pool, this model avoids clonal selection artifacts and better replicates the genetic heterogeneity of tumor cells, making it suitable for pooled functional assays such as proliferation, migration, and protein interaction studies.
NCI-H1299 is a metastatic lung adenocarcinoma cell line derived from a lymph node metastasis, widely used to study NSCLC progression and invasion. Its aggressive in vitro behavior and relevance to late-stage disease make it an ideal host for interrogating genes implicated in tumor metastasis. The CCDC167 knockout in this context allows direct assessment of the gene??s role in metastatic processes within a clinically meaningful cellular background.
The CCDC167 protein features a coiled-coil domain, a motif frequently involved in protein-protein interactions and multiprotein complex assembly. Although its specific binding partners and downstream signaling pathways remain unknown, this structural feature suggests a role in organizing cellular complexes that may govern processes including signal transduction, cytoskeletal dynamics, or membrane trafficking. Loss-of-function analysis using this knockout model enables de novo discovery of CCDC167 interactors and functional pathways through comparative proteomic and phenotypic approaches.
In NCI-H1299 cells, CCDC167 disruption provides a direct strategy to evaluate its contribution to metastatic phenotypes such as cell motility, invasiveness, and anchorage-independent growth. Because the polyclonal knockout pool preserves cell-to-cell variation, any dominant CCDC167-dependent phenotypes are less likely to be confounded by secondary genetic changes that can arise during single-cell cloning. Thus, the model supports robust genotype-phenotype correlations in a disease-relevant system.
Key applications include functional characterization via cell proliferation, wound-healing, and Matrigel invasion assays, validated by western blotting and RT-qPCR. Immunofluorescence studies can reveal alterations in CCDC167 subcellular distribution, while co-immunoprecipitation and mass spectrometry enable identification of novel interacting partners. The knockout also supports drug response profiling and genetic modifier screens. For further details or custom editing requests, please contact Ascent Research.
