The CCDC24 Knockout HEK293T Polyclonal Cells product provides a ready-to-use, CRISPR/Cas9-edited polyclonal population derived from the widely utilized HEK293T cell line, in which the CCDC24 locus has been disrupted. This pooled knockout format offers a heterogeneous mixture of edited cells, each harboring distinct editing outcomes at the CCDC24 genomic locus, resulting in a loss-of-function model amenable to bulk biochemical and cell-based analyses. The polyclonal nature maintains genetic diversity while enabling robust assessment of CCDC24-dependent phenotypes at the population level, bypassing the single-clone selection process typically required for monoclonal knockout lines.
HEK293T cells are human embryonic kidney epithelial cells that stably express the SV40 large T antigen, which permits episomal replication of plasmids containing the SV40 origin of replication and confers high transfectability and robust protein expression capabilities. This host cell line is a cornerstone of mammalian cell biology research, employed extensively for heterologous protein production, lentiviral and retroviral packaging, and signal transduction studies owing to its ease of manipulation and well-characterized biochemical properties. The HEK293T background thus provides a permissive cellular environment for interrogating gene function through gene disruption approaches.
CCDC24 encodes a predicted coiled-coil domain-containing protein, a structural motif known to facilitate protein-protein interactions and often involved in the assembly of multi-protein complexes and scaffolding functions. Although the precise molecular role of CCDC24 remains undefined, coiled-coil domains are characteristic of diverse proteins participating in processes such as intracellular trafficking, cytoskeletal organization, and signal transduction. Current knowledge does not identify specific upstream regulators, downstream targets, or interaction partners for CCDC24, underscoring the need for functional characterization in a tractable cellular system such as HEK293T. The absence of characterized signaling network connections positions these knockout cells as a key tool for de novo pathway discovery.
In the HEK293T context, disruption of CCDC24 may reveal impacts on cellular processes mediated by coiled-coil domain-containing complexes, particularly given the cell line??s utility in signaling studies and its robust capacity for protein interaction analyses. The polyclonal knockout population is well-suited for assays that measure overall changes in cell behavior, such as proliferation, migration, or interaction with common binding partners identified through co-immunoprecipitation. Because HEK293T cells support high-level expression of exogenous proteins, this model also enables functional complementation experiments where wild-type or mutant CCDC24 can be reintroduced to confirm phenotype specificity.
This CCDC24 knockout product supports a range of research applications, including functional characterization of the CCDC24 gene, validation of knockout efficiency via Western blotting and RT-qPCR, investigation of protein-protein interaction networks through co-immunoprecipitation and immunofluorescence, and assessment of cellular phenotypes such as proliferation alterations. The representative assays??Western blotting, RT-qPCR, immunofluorescence, co-immunoprecipitation, and proliferation assays??provide researchers with a comprehensive toolkit for exploring the biological role of CCDC24 in a human cell background. For further details or technical inquiries, please contact Ascent Research.
