This product is a CRISPR/Cas9-edited polyclonal knockout cell population targeting the human CCDC24 gene in HeLa cells. The polyclonal mixture contains diverse edited alleles, providing a robust loss-of-function model for studying centrosome biology, cell cycle regulation, and primary cilium assembly.
HeLa cells are an HPV18-positive human cervical adenocarcinoma epithelial line widely used in cancer biology, cell cycle, and gene expression studies. Their rapid growth and well-characterized signaling pathways make them ideal for investigating centrosome dynamics and ciliogenesis with CRISPR-mediated gene disruption.
CCDC24 encodes a centrosomal protein that scaffolds centriole duplication and microtubule anchoring. It is activated downstream of PLK4 kinase and functions upstream of centriolar assembly factors CEP135 and CEP152. CCDC24 interacts with CEP63, CDK5RAP2, and microtubules to maintain centrosome integrity. The protein also localizes to basal bodies for primary cilium formation, mediating Hedgehog signaling from the PTCH1-SMO complex to GLI transcription factors. Knockout of CCDC24 disrupts these complexes, leading to aberrant centriole numbers, defective microtubule organization, and impaired ciliary-dependent signaling.
In HeLa cells, CCDC24 knockout causes centrosome amplification, multipolar spindles, and chromosome mis-segregation, resulting in mitotic catastrophe or cell cycle arrest. Additionally, impaired ciliogenesis attenuates Hedgehog pathway activity, linking CCDC24 to both proliferation control and paracrine signaling. The HeLa background provides a reproducible epithelial platform for high-throughput screening of centrosome-targeted compounds and functional ciliopathy assays, making this model valuable for dissecting pathologies such as cancer, ciliopathies, microcephaly, and polycystic kidney disease.
This polyclonal knockout population is suitable for immunofluorescence analysis of centrosome and cilium markers, ciliogenesis induction and enumeration, cell cycle flow cytometry, and mitotic index scoring. Co-immunoprecipitation with CEP152, CEP135, or PLK4 clarifies interaction networks, while western blotting for CDK1 and Cyclin B1 assesses cell cycle status. Migration and invasion assays further enable cancer biology studies. For drug target validation or custom applications, contact Ascent Research.
