The CCDC3 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population targeting the CCDC3 gene in the near-haploid HAP1 cell line. This loss-of-function model provides a genetically diverse cell pool for functional genomics studies of adipogenesis, lipid metabolism, and insulin signaling, without clonal selection bias. The knockout cells serve as a valuable tool for investigating CCDC3-related pathways and disorders such as familial partial lipodystrophy type 6.
HAP1 is a near-haploid human cell line derived from KBM-7 chronic myeloid leukemia cells, characterized by fibroblast morphology and rapid proliferation. Its haploid genome simplifies genetic manipulation and phenotypic analysis, making it an ideal host for CRISPR-mediated knockout studies. HAP1 cells are widely used in functional genomics screens and mechanistic studies, providing a robust and reproducible system for probing gene function in a human context.
CCDC3 encodes a coiled-coil domain protein involved in adipocyte differentiation and lipid storage. It interacts with nuclear envelope components such as lamin A/C and modulates the transcriptional activity of PPAR?? and C/EBP??. CCDC3 is activated downstream of insulin and PPAR?? and regulates the expression of SREBP1c, FABP4, ADIPOQ, and GLUT4. These factors control lipogenesis, fatty acid transport, adipokine secretion, and glucose uptake, collectively governing adipogenic commitment and metabolic homeostasis.
In HAP1 cells, CCDC3 disruption is expected to impair adipogenic gene expression and lipid accumulation, consistent with its role in adipogenesis. The polyclonal knockout population captures a range of loss-of-function alleles, enabling the study of both complete and partial gene disruption effects. This model is particularly useful for dissecting early signaling events in adipogenesis and the interplay between CCDC3, the nuclear envelope, and insulin signaling, taking advantage of the HAP1 cell line??s expression of relevant molecular partners.
The CCDC3 Knockout HAP1 Polyclonal Cells support diverse functional assays, including Oil Red O staining for lipid droplet formation, RT-qPCR analysis of adipogenic markers (e.g., FABP4, ADIPOQ), western blotting for insulin signaling components (e.g., phospho-AKT, GLUT4), and immunofluorescence of nuclear envelope proteins. Metabolic flux analysis can be applied to measure lipid uptake and synthesis. These cells are applicable to research on adipogenesis, lipodystrophy, insulin resistance, and metabolic syndrome. For additional information, please contact Ascent Research.
