The CCDC6 Knockout HGC-27 Polyclonal Cells product consists of a heterogeneous population of HGC-27 gastric carcinoma cells engineered via CRISPR/Cas9-mediated disruption of the CCDC6 gene. This polyclonal knockout cell pool retains the genetic diversity resulting from multiple editing events, enabling robust loss-of-function studies without clonal selection. The cells are provided as a ready-to-use reagent for investigating tumor suppressor functions and DNA damage response pathways in a gastric cancer background.
The parental HGC-27 cell line is a human gastric carcinoma line originally derived from the lymph node metastasis of a patient with gastric cancer. HGC-27 cells exhibit typical epithelial morphology and are widely employed as a model system for studying gastric cancer biology, including metastatic properties, signaling dysregulation, and therapeutic responses. This host background is particularly relevant for evaluating the role of tumor suppressors in advanced, metastatic gastric malignancies.
CCDC6 encodes a coiled-coil domain-containing protein that functions as a tumor suppressor integral to the ATM-mediated DNA damage response. Upon DNA double-strand breaks, CCDC6 is phosphorylated by ATM kinase and facilitates the activation of downstream effectors including CHEK2, TP53, and H2AFX. CCDC6 also interacts with CREB1 to regulate transcription of apoptotic regulators such as BCL2 family members and caspase activation cascades. Consequently, CCDC6 knockout impairs ATM signaling, reduces DNA damage-induced apoptosis, and dysregulates CREB1-dependent gene expression, fostering genomic instability and enhanced cell survival.
In the context of HGC-27 gastric carcinoma cells, loss of CCDC6 function is predicted to exacerbate malignant phenotypes by compromising the cellular DNA damage checkpoint and apoptotic machinery. This knockout model thus provides a physiologically relevant platform to dissect how CCDC6 inactivation contributes to gastric cancer progression, chemoresistance, and genomic instability. The polyclonal nature of the product allows researchers to analyze population-level effects while avoiding artifacts from clonal selection.
This polyclonal CCDC6 knockout cell pool is suitable for diverse experimental applications including tumor suppressor gene studies, DNA damage signaling analyses, and chemosensitivity profiling. Researchers can assess CCDC6-dependent responses using western blotting, RT-qPCR, immunofluorescence detection of ??-H2AX foci, flow cytometry for apoptosis and cell cycle distribution, colony formation assays, comet assays, and viability-based drug sensitivity screens. The model supports both mechanistic and translational investigations in gastric cancer research. For additional product details or technical inquiries, please contact Ascent Research.
