The CCDC71 Knockout HEK293T Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal cell population derived from the HEK293T human embryonic kidney cell line, designed to disrupt the CCDC71 gene. This loss-of-function model is generated through CRISPR/Cas9-mediated gene targeting, yielding a mixed population of cells with heterogeneous gene editing events. The polyclonal format provides a robust and versatile tool for studying CCDC71 function without the clonal selection bottleneck. The product is supplied as a ready-to-use population of knockout cells, suitable for direct experimental applications in molecular and cellular biology.
HEK293T cells are a widely utilized human embryonic kidney epithelial cell line that stably expresses the SV40 large T antigen. This characteristic enhances the episomal replication of plasmids containing the SV40 origin of replication, thereby enabling high-level transient protein expression and efficient production of lentiviral and retroviral particles. The cells are highly transfectable and maintain robust growth in standard culture conditions. Their epithelial origin and well-characterized signaling networks make them an ideal host for investigating gene function in both normal and disease-relevant contexts, particularly in cancer biology and signal transduction studies.
The CCDC71 gene encodes a putative coiled-coil domain-containing protein of largely unknown biological function. Coiled-coil domains are structural motifs that commonly mediate protein-protein interactions, suggesting that CCDC71 may participate in multiprotein complexes involved in cellular signaling, structural organization, or transcriptional regulation. Although its specific upstream regulators and downstream effectors remain to be identified, CCDC71 has been linked to hepatocellular carcinoma, where it may act as a potential tumor suppressor. It is presumed to interact with other coiled-coil domain proteins, potentially influencing pathways governing cell proliferation and apoptosis. Further mechanistic studies are needed to elucidate its precise role in these processes.
In the HEK293T background, knockout of CCDC71 provides a physiologically relevant platform for dissecting its function in an epithelial cell environment. HEK293T cells express many endogenous signaling components, allowing the study of CCDC71 within a context that models aspects of kidney epithelial biology and oncogenic transformation. Because HEK293T cells are permissive for transfection and viral transduction, this knockout model can be readily combined with exogenous expression constructs, shRNA, or pathway reporters to interrogate CCDC71??s involvement in cell cycle progression, survival, and motility. The polyclonal nature of the knockout population also mitigates clonal artifacts, providing a more representative assessment of gene disruption effects.
This polyclonal knockout cell population is suited for functional genomics screens, protein-protein interaction analyses, and cancer-focused investigations. Researchers can confirm CCDC71 knockout via Western blotting and RT-qPCR, while immunofluorescence and co-immunoprecipitation probe its localization and binding partners. Proliferation, apoptosis, and migration assays enable direct assessment of CCDC71??s impact on tumorigenic phenotypes, particularly in hepatocellular carcinoma research. The knockout cells also serve as a control for complementation studies, enabling structure-function analysis of CCDC71 domains. For further information or support, please contact Ascent Research.
