The CCDC71L Knockout AGS Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the AGS human gastric adenocarcinoma cell line. These cells feature targeted disruption of the CCDC71L gene, encoding a coiled-coil domain protein of unknown function. This heterogeneous loss-of-function model enables study of CCDC71L-dependent phenotypes in a gastric epithelial context, providing a tool for functional genomics research.
The AGS cell line (ATCC CRL-1739) originates from a poorly differentiated gastric adenocarcinoma in a 54-year-old woman. AGS cells display adherent epithelial morphology and serve as a model for gastric epithelial biology, H. pylori infection, and cancer signaling studies, making them suitable for investigating genes relevant to gastric adenocarcinoma.
CCDC71L contains coiled-coil domains that mediate protein-protein interactions, but its biological function is largely uncharacterized. Coiled-coil domains are involved in cytoskeletal organization and signaling complex assembly, suggesting CCDC71L may participate in these processes. Its knockout could disrupt protein interaction networks, potentially affecting cytoskeletal dynamics or signaling pathways such as Wnt signaling in gastric epithelial cells. The loss-of-function model thus offers a means to explore CCDC71L’s role in cellular architecture and signal transduction.
Knocking out CCDC71L in AGS cells provides a relevant platform to investigate its potential contributions to gastric adenocarcinoma phenotypes, including proliferation, migration, and invasion. The AGS background enables studies of host-pathogen interactions with H. pylori, a gastric cancer risk factor. The polyclonal nature avoids clonal biases, yielding a more representative assessment of CCDC71L’s functional impact.
The cells are suitable for Western blotting and RT-qPCR to confirm CCDC71L loss, and proliferation, migration, and invasion assays to assess phenotypic changes. Co-immunoprecipitation can identify protein interaction partners, while RNA-seq permits transcriptome-wide analysis. These tools support functional genomics screening for gastric cancer-related genes. For further details, contact Ascent Research.
