CCDC71L Knockout HAP1 Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population designed to disrupt the CCDC71L gene in the HAP1 human cell line. This genetically heterogeneous pool, generated via CRISPR-mediated gene disruption, provides a powerful tool for investigating the functional role of the poorly characterized coiled-coil domain-containing protein CCDC71L. The knockout model enables loss-of-function studies in a near-haploid background, facilitating genetic screens and protein interaction analyses, and is well-suited for researchers exploring signal transduction and protein scaffolding mechanisms.
The host HAP1 cell line is a near-haploid adherent cell line derived from the KBM-7 chronic myeloid leukemia (CML) line, which retains the oncogenic BCR-ABL1 fusion kinase. Its near-haploid karyotype simplifies genetic manipulation and phenotypic interpretation, making it a preferred platform for CRISPR-based functional genomics. HAP1 cells maintain key signaling pathways characteristic of CML, providing a disease-relevant context for studying genes involved in leukemogenesis and drug resistance.
CCDC71L encodes a coiled-coil domain-containing protein whose function remains largely uncharacterized. Coiled-coil domains are known to mediate protein-protein interactions, and CCDC71L is predicted to engage in homotypic interactions with other coiled-coil proteins. Potential interacting partners may include centrosomal or cytoskeletal components, suggesting roles in subcellular organization or signal transduction pathways. Although upstream regulators and downstream targets are not yet defined, CCDC71L may be implicated in responses to cell cycle cues or cellular stress. The knockout model provides a clean background to dissect these interactions using co-immunoprecipitation and mass spectrometry.
The introduction of a CRISPR-mediated CCDC71L disruption in HAP1 cells creates a versatile model for exploring the gene’s contribution to cellular processes in a leukemia-derived context. The near-haploid nature of HAP1 cells enhances the efficiency of loss-of-function screening and allows for unbiased identification of genetic interactions. Given the potential cancer associations hinted at by genomic studies, this knockout cell population is a valuable resource for examining how CCDC71L influences proliferation, apoptosis, or cytoskeletal dynamics. By leveraging the BCR-ABL1-positive background, researchers can assess interplay with leukemogenic signaling.
Researchers can utilize these polyclonal knockout cells in a wide array of functional assays, including Western blotting and RT-qPCR to confirm gene disruption and downstream effects, RNA-sequencing for transcriptome-wide profiling, and immunofluorescence to examine protein localization. Co-immunoprecipitation experiments can be employed to identify and validate CCDC71L interaction partners, while flow cytometry, proliferation, and apoptosis assays offer quantitative phenotypic readouts. The cell pool format provides a robust system for pooled CRISPR screens and high-throughput studies. For additional technical information and ordering details, please contact Ascent Research.
