The CCDC71L Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from human embryonic kidney HEK293T cells, featuring targeted disruption of the CCDC71L gene. This product provides a heterogeneous pool of edited cells for loss-of-function analysis of the coiled-coil domain-containing protein CCDC71L, avoiding clonal selection bias. The polyclonal format preserves population diversity and allows robust screening of gene function in a well-characterized host background.
HEK293T cells constitutively express the SV40 large T antigen, permitting episomal plasmid amplification and high protein yields. They are a preferred platform for recombinant protein expression, lentivirus production, and gene delivery owing to excellent transfectability and consistent growth. As an embryonic kidney-derived epithelial line, HEK293T is widely adopted for studies of signaling, adhesion, and intracellular trafficking.
CCDC71L encodes a protein with predicted coiled-coil domains, suggesting a scaffold or adaptor role in protein-protein interactions. It may self-associate and engage other coiled-coil proteins, potentially linking to actin filaments and cytoskeletal organization. Upstream regulation involves generic transcription factors and cellular stress signals, while downstream effects might modulate cell proliferation or adhesion. Disruption of CCDC71L likely interferes with the assembly of these interaction networks, providing a tool to dissect the functional significance of coiled-coil-mediated complexes.
The knockout in HEK293T cells offers a simplified model to study CCDC71L without the confounding variables of primary cell differentiation. The high transfection efficiency facilitates rescue experiments with wild-type or mutant CCDC71L constructs, enabling structure-function analyses. Because the cells are polyclonal, they represent a population-average effect, reducing the risk of clonal artifacts. This model is advantageous for exploring how loss of a putative scaffold protein impacts cellular architecture and stress responses in an epithelial context frequently used for cancer biology research.
Researchers can employ these cells in co-immunoprecipitation to identify CCDC71L interaction partners, immunoblotting to confirm protein knockout, and immunofluorescence to visualize changes in adhesion or cytoskeletal markers. Proliferation, migration, and invasion assays can assess the gene??s role in cancer-like phenotypes. Transcriptomic profiling via RNA-seq may reveal downstream gene expression changes and pathway alterations. These applications position the knockout cells as valuable tools in functional genomics and coiled-coil domain research. For technical inquiries and custom knockout cell services, please contact Ascent Research.
