The CCDC82 Knockout HAP1 Polyclonal Cells product provides a ready-to-use CRISPR/Cas9-edited polyclonal cell population targeting the human CCDC82 gene in the near-haploid HAP1 cell line. This knockout pool, generated through CRISPR/Cas9-mediated gene disruption, serves as a loss-of-function model to investigate the biological roles of coiled-coil domain-containing protein 82 (CCDC82). The polyclonal format captures a diverse array of editing events across the cell population, enabling robust functional studies without the need for single-cell cloning. This product is suitable for researchers exploring CCDC82 function in spermatogenesis, cytoskeletal organization, and cancer cell biology.
The host HAP1 cell line is a near-haploid human line derived from the KBM-7 chronic myeloid leukemia line. Its haploid karyotype makes HAP1 cells a powerful system for genetic perturbation, requiring disruption of only one allele for functional knockout. This characteristic is valuable for functional genomics screens, drug target validation, and pathway analysis. The HAP1 background retains cancer-relevant signaling pathways, allowing study of CCDC82 disruption effects on proliferation and morphology despite its typical association with spermatogenesis.
CCDC82 encodes a coiled-coil domain protein with predicted roles in spermatogenesis and cytoskeletal dynamics. It is thought to interact with coiled-coil domain and microtubule-associated proteins, contributing to flagellar assembly and axonemal structure. Pathway components include SPAG6 and AKAP4, critical for sperm motility and cilia. Upstream regulators are unknown, but testis-specific transcription factors may control expression. In HAP1 cells, CCDC82 knockout may disrupt structural protein interactions, altering cytoskeletal organization and cell cycle progression.
Although the precise function of CCDC82 in leukemia-derived HAP1 cells is not well characterized, the knockout model provides a unique opportunity to dissect its roles in cancer cell biology. Near-haploid HAP1 cells facilitate unambiguous genotype-phenotype correlations, allowing researchers to assess how loss of CCDC82 affects proliferation, colony formation, and cellular morphology. Given the protein??s coiled-coil motifs, it may serve as a scaffold for signaling complexes relevant to both normal spermatogenesis and aberrant processes in cancer. This model can help uncover potential moonlighting functions of CCDC82 in mitotic cells, expanding the understanding of its biological significance beyond reproductive tissues.
This polyclonal knockout cell pool is ideally suited for a range of downstream applications, including functional genomics studies, protein interaction analyses, and cancer cell biology research. Researchers can employ standard assays such as Western blotting and RT-qPCR to confirm gene disruption at the protein and mRNA levels, respectively. Immunofluorescence microscopy facilitates visualization of cytoskeletal changes, while cell proliferation and colony formation assays quantify growth alterations. These CCDC82 knockout cells offer a versatile platform for investigating the interplay between coiled-coil domain proteins and cellular architecture. For additional information, please contact Ascent Research.
