The CCDC82 Knockout HT29 Polyclonal Cells product is a CRISPR/Cas9-edited polyclonal population with targeted disruption of CCDC82 in the HT29 colorectal adenocarcinoma cell line. This heterogeneous knockout model preserves polyclonal diversity, avoiding clonal artifacts and enabling robust pooled functional analyses. The polyclonal format is well-suited for assays that require population-level representation and facilitates direct comparison with parental HT29 cells.
HT29 cells are derived from a female patient with Dukes’ stage B colorectal adenocarcinoma and exhibit chromosomal instability along with well-characterized mutations in APC, TP53, and KRAS. This line retains the ability to differentiate into enterocyte-like and mucin-secreting goblet cells, making it a prominent model for intestinal epithelial cell biology, including studies of differentiation, barrier function, and colorectal cancer progression.
CCDC82 encodes a predicted coiled-coil domain-containing protein that is hypothesized to function as a molecular scaffold within signaling and cytoskeletal networks. It may interact with centrosomal or ciliary proteins and is potentially regulated by RAS/MAPK and PI3K/AKT pathways. CCDC82 has been associated with Wnt/??-catenin signaling, where key components include APC, CTNNB1 (??-catenin), AXIN, DVL, and GSK3B. Knockout of CCDC82 is anticipated to alter cell cycle progression, cytoskeletal dynamics, and ??-catenin-mediated transcription, although direct interacting partners and downstream effectors remain largely unknown.
Given that HT29 cells harbor an APC mutation leading to constitutive Wnt pathway activation, disruption of CCDC82 provides a powerful system to dissect its role in transmitting or modulating oncogenic signals. The polyclonal knockout approach allows assessment of CCDC82-dependent phenotypes such as proliferation, migration, invasion, and anchorage-independent growth without clonal selection bias. This model is particularly relevant for investigating how coiled-coil scaffolding proteins organize multiprotein complexes that drive malignant behavior in colorectal cancer.
Researchers can utilize these cells in a wide range of experimental workflows, including Western blotting and RT-qPCR for knockout validation and target expression analysis, MTT or BrdU proliferation assays, transwell migration and invasion assays, colony formation assays, and apoptosis detection. Transcriptomic analysis via RNA-seq can uncover global gene expression changes, while immunofluorescence enables visualization of cytoskeletal reorganization. Luciferase-based Wnt reporter assays directly measure ??-catenin activity. This product is ideal for mechanistic studies of coiled-coil proteins in tumorigenesis, drug target identification, and preclinical drug screening. For further information, please contact Ascent Research.
