CCDC82 Knockout Huh-7 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human hepatocellular carcinoma line Huh-7. This product provides heterogeneous disruption of the CCDC82 gene, which encodes a centriolar protein essential for centrosome function and ciliogenesis. The polyclonal format ensures a diverse pool of loss-of-function mutations, enabling robust gene function analysis without the biases of clonal selection.
The host Huh-7 cell line is a well-characterized hepatocellular carcinoma model with epithelial morphology and tumorigenic properties. It retains molecular features of liver cancer, including dysregulated signaling pathways, making it an appropriate system for probing CCDC82??s role in hepatocellular carcinoma. Huh-7 cells are widely used in cancer biology for studies on proliferation, metastasis, and drug response.
CCDC82 localizes to centrioles and is required for centriole duplication and primary cilium assembly. It functions downstream of the PLK4 and CDK1 kinases and interacts with centriolar proteins CEP152, CEP63, CEP135, and CPAP. CCDC82-mediated ciliogenesis is essential for Hedgehog signaling, where ciliary SMO activation relieves PTCH1-mediated repression, leading to GLI1 and GLI2 transcription factor activation and target gene expression. Knockout of CCDC82 disrupts cilia formation, thereby impairing Hedgehog pathway output and downstream cellular responses. Additional regulators like AURKA and CEP192 further coordinate these processes, underscoring the intricate connection between centrosome biology and signal transduction.
In liver cancer, aberrant centrosome numbers and ciliary dysfunction are associated with tumor progression, metastasis, and chemoresistance. This knockout model allows investigation of how CCDC82 loss alters hepatocellular carcinoma cell behavior, including cell cycle progression, migration, and drug sensitivity. It also provides a platform to study ciliopathy-related mechanisms, as defects in cilia assembly are linked to several liver-affected syndromes. Consequently, the CCDC82 knockout Huh-7 cells serve as a valuable tool for bridging rare disease biology and hepatocellular carcinoma research.
Typical applications include immunofluorescence staining for centriole and cilia markers (??-tubulin, centrin, acetylated ??-tubulin, Arl13b), western blotting, and RT-qPCR for Hedgehog targets like GLI1. Flow cytometry can assess cell cycle alterations, and drug response assays may reveal therapeutic vulnerabilities. The polyclonal knockout population is ideal for functional screens and dose-response studies. For additional details, please contact Ascent Research.
