The CCDC82 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting the coiled-coil domain-containing protein 82 (CCDC82) gene. This gene-disrupted pool, derived from Jurkat T cells, provides a heterogeneous loss-of-function model suitable for pooled studies. The polyclonal format includes diverse mutations at the target locus, enabling robust functional assessment without clonal artifacts. These cells are optimized for transcriptomic profiling, protein analysis, and live-cell assays.
The Jurkat E6.1 host cell line is a widely used human T lymphocyte model isolated from the peripheral blood of an acute T cell leukemia patient. Growing in suspension and expressing CD4, these cells are instrumental for studying adaptive immune responses and T cell receptor (TCR) signaling. Jurkat cells are routinely employed to dissect mechanisms of T cell activation, proliferation, and apoptosis, and their leukemic background provides a relevant context for examining gene function in lymphocyte biology and oncogenesis.
CCDC82 encodes a coiled-coil domain protein, a structural motif commonly involved in mediating protein?Cprotein interactions and complex assembly. While its molecular partners and signaling role are largely uncharacterized, coiled-coil proteins frequently act as scaffolds or adaptors. In Jurkat T cells, CCDC82 may influence TCR-proximal signaling, cytoskeletal organization, or transcriptional regulation. The polyclonal knockout pool enables unbiased identification of interacting partners and downstream effects through proteomic and genomic comparisons.
This CCDC82 knockout model provides a flexible platform for investigating the gene??s function in T lymphocyte biology. Since CCDC82 is poorly understood, the model allows association of its loss with specific phenotypes in adaptive immunity and leukemic T cell behavior, such as changes in activation markers, cytokine secretion, or survival. The polyclonal composition reduces clonal selection bias, supporting generalizable conclusions about gene function in a heterogeneous cell population.
Typical applications include functional genomics screens, protein interaction profiling, and phenotypic characterization using Western blotting for CCDC82, RT-qPCR, RNA-seq, and flow cytometry of surface markers like CD69 and CD25. Proliferation and apoptosis assays extend these studies to cell cycle and death pathways. These cells are also compatible with pharmacological inhibitors or TCR stimulation to place CCDC82 within signaling networks. For technical inquiries, please contact Ascent Research.
