The CCDC82 Knockout MES-OV Polyclonal Cells provide a CRISPR/Cas9-edited polyclonal knockout cell population targeting the human CCDC82 gene within the MES-OV ovarian carcinoma host line. This model enables loss-of-function studies of the coiled-coil domain-containing protein CCDC82, implicated in scaffolding signaling complexes. The polyclonal format yields a genetically heterogeneous knockout pool suitable for direct functional analyses without clonal isolation, maintaining population-level variation.
MES-OV, a widely utilized human high-grade serous ovarian adenocarcinoma cell line, serves as a pathophysiologically relevant host for studying ovarian cancer biology, including tumorigenesis, metastasis, and drug resistance. These cells retain dysregulated Wnt/??-catenin signaling, which is central to their proliferative and migratory behavior, and thus provide an appropriate context for interrogating CCDC82 function.
CCDC82 encodes a coiled-coil protein thought to scaffold Wnt/??-catenin pathway components. It physically interacts with ??-catenin and AXIN, and associates with cytoskeletal regulators. In response to Wnt ligands such as Wnt3a, Frizzled receptors and Dishevelled stabilize ??-catenin, which then partners with TCF/LEF transcription factors to activate target genes. CCDC82 may enhance this transcriptional program by promoting ??-catenin/TCF complex activity, leading to upregulation of MYC, CCND1, and MMP9??genes involved in cell cycle progression and cell migration. Thus, CCDC82 functions as a positive modulator of proliferation and invasion via the Wnt cascade.
In ovarian carcinoma, CCDC82 dysregulation is associated with aggressive disease and poor outcome. Knockout in MES-OV cells permits dissection of its role in Wnt-driven oncogenic phenotypes, such as anchorage-independent growth, epithelial-mesenchymal transition, and chemoresistance. The polyclonal model captures phenotypic heterogeneity, reflecting intratumoral diversity and potential resistance mechanisms, and allows investigation of CCDC82-dependent cytoskeletal dynamics and focal adhesion changes.
Key applications include western blotting and RT-qPCR for knockout confirmation, wound healing and transwell invasion assays for migration/invasion phenotyping, and TOP/FOP-Flash reporter assays to measure ??-catenin-mediated transcription. The cells are suited for drug target screening, pathway dissection, and identification of synthetic lethal interactions in ovarian and gastric cancer. For further details and ordering, please contact Ascent Research.
