The CCDC85C Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population targeting the CCDC85C gene in the HEK293T human embryonic kidney cell line. As a polyclonal pool, this product contains a heterogeneous mixture of edited alleles, avoiding the clonal selection biases of monoclonal lines and providing a robust loss-of-function model. CCDC85C encodes a predicted coiled-coil domain-containing protein with currently uncharacterized function, making this tool valuable for initial functional investigations.
HEK293T cells are immortalized human embryonic kidney cells expressing the SV40 large T antigen, which enables episomal replication of plasmids bearing the SV40 origin. This feature underpins their exceptional utility for recombinant protein expression, lentiviral packaging, and transient transfection. Derived from adenovirus type 5 DNA integration, these cells are highly transfectable and support a wide range of biochemical and imaging assays, forming an ideal background for gene-editing and functional genomics studies.
The CCDC85C protein contains coiled-coil motifs, known to mediate protein?Cprotein interactions and complex assembly. However, its specific binding partners, regulatory inputs, and downstream effectors have not been identified. CRISPR-mediated disruption in this polyclonal knockout population provides a clean experimental system to uncover CCDC85C??s role in cellular processes, potentially involving scaffolding functions or trafficking. Unbiased proteomic approaches and genetic screens can be applied to map its interaction network and functional dependencies.
In the HEK293T background, the knockout cells enable complementation studies using SV40-based episomal vectors to reintroduce wild-type or mutant CCDC85C, allowing rescue experiments and structure-function analysis. The polyclonal format offers a heterogeneous pool that better reflects variable editing outcomes, enhancing statistical robustness in pooled screens and reducing the impact of off-target effects. This model is well-suited for initial phenotypic characterization of an uncharacterized gene in a genetically tractable human cell context.
Researchers can utilize these knockout cells in assays such as western blotting and RT-qPCR for target verification, immunofluorescence for localization studies, and co-immunoprecipitation-mass spectrometry to identify CCDC85C interactors. Functional assays including proliferation, migration, and reporter gene analyses facilitate phenotype discovery. The polyclonal pool serves as a flexible resource for dissecting the biological role of CCDC85C in human cell biology. For further technical details, please contact Ascent Research.
