CCDC85C Knockout Huh-7 Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population wherein the CCDC85C gene was disrupted to create a loss-of-function model. This product comprises a heterogeneous pool of Huh-7 hepatocellular carcinoma cells, each carrying distinct CCDC85C mutations, thus avoiding clonal bias and enabling functional studies in a physiologically relevant cell background. The polyclonal format is ideal for investigating gene function without the confounds of single-cell adaptation. Utilizing CRISPR/Cas9-mediated gene disruption, researchers can probe the consequences of CCDC85C loss in malignant hepatocytes, supporting diverse experimental designs from pooled screens to individual assay validation.
The host Huh-7 cell line was established from a well-differentiated hepatocellular carcinoma of a Japanese male patient and is extensively used for liver cancer biology and hepatitis C virus (HCV) research. Huh-7 cells maintain hepatocyte characteristics and are naturally permissive to HCV replication, making them a robust model for exploring virus-host interactions and oncogenic signaling pathways. This well-characterized cell line provides a clinically pertinent system to dissect the role of CCDC85C within the context of hepatic malignancy and viral pathogenesis.
CCDC85C encodes a coiled-coil domain-containing protein, a structural feature frequently involved in mediating protein-protein interactions, yet its specific molecular functions and interaction partners remain undefined. No canonical upstream regulators, downstream effectors, or pathway affiliations have been identified for CCDC85C. Consequently, this knockout model is a pivotal resource for de novo functional discovery. By eliminating CCDC85C expression in Huh-7 cells, researchers can perform unbiased proteomic and transcriptomic analyses to delineate its interactome and its impact on cellular signaling networks.
In the malignant hepatocyte background, CCDC85C disruption enables systematic investigation of its potential roles in proliferation control, apoptosis, or viral host factor modulation. The Huh-7 model??s HCV permissiveness allows direct assessment of whether CCDC85C influences viral life cycle steps such as replication, entry, or egress. Because the line originates from a male patient, it may also provide insights into sex-specific aspects of HCC biology. This knockout system thus serves as a versatile platform for elucidating CCDC85C??s contributions to both intrinsic tumor cell properties and pathogen-host dynamics.
Key applications include functional genomics assays, HCV replication studies, and cancer cell biology investigations. Standard techniques such as western blotting, RT-qPCR, and immunofluorescence are routinely used to confirm CCDC85C loss and monitor cellular responses. Cell proliferation assays quantify growth effects, while luciferase-based HCV replicon systems enable precise measurement of viral replication. The polyclonal nature also permits pooled CRISPR screens and drug-response profiling. This product is a valuable tool for advancing understanding of CCDC85C in liver cell biology. For technical inquiries and ordering, contact Ascent Research.
