The CCDC85C Knockout NCI-H1299 Polyclonal Cells product offers a CRISPR/Cas9-edited polyclonal knockout cell population, achieved by disrupting the CCDC85C gene within the NCI-H1299 lung carcinoma epithelial cell line. This polyclonal pool harbors a diverse collection of loss-of-function alleles, providing a robust experimental system that minimizes clonal biases. Suitable for a range of downstream assays, these cells facilitate rigorous functional investigations of CCDC85C.
The NCI-H1299 host cell line is a widely used model of non-small cell lung cancer (NSCLC), originally isolated from a metastatic lymph node of a lung adenocarcinoma patient. It exhibits a homozygous deletion of TP53 and wild-type KRAS and EGFR alleles, representing a p53-deficient background pertinent to aggressive lung adenocarcinoma. The line??s epithelial nature and metastatic origin make it particularly valuable for studying cancer cell invasion and migration.
CCDC85C encodes a coiled-coil domain-containing scaffold protein that orchestrates cytoskeletal organization and cell adhesion. It interacts with DISC1, CCDC85A, CCDC85B, and centrosomal proteins, and integrates into Wnt signaling by associating with ??-catenin and GSK3??. Although its upstream regulators and downstream targets remain largely unknown, CCDC85C is thought to modulate actin dynamics. Disruption of CCDC85C in NCI-H1299 cells is expected to impair these protein complexes, leading to defective migration and altered signaling cascades critical for tumor behavior.
Loss of CCDC85C in the NCI-H1299 background provides a unique model to dissect its contribution to NSCLC metastasis. Given the cell line??s p53 loss and origin from a metastatic site, this knockout enables analysis of CCDC85C-dependent cytoskeletal remodeling and adhesion dynamics during invasive processes. Exploring the interplay between CCDC85C, ??-catenin, and GSK3?? is particularly relevant, as aberrant Wnt signaling is a hallmark of lung cancer progression.
This polyclonal knockout product supports diverse research applications, including transwell invasion and wound healing migration assays, co-immunoprecipitation for mapping protein interactions, and drug response profiling. Transcriptomic studies via RNA-seq, combined with standard techniques such as western blotting, RT-qPCR, and immunofluorescence, permit comprehensive characterization of knockout effects. Cell viability and apoptosis assays can also be employed. For additional information, please contact Ascent Research.
