The CCDC90B Knockout AGS Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the AGS human gastric adenocarcinoma cell line. This pooled model introduces loss-of-function mutations in the CCDC90B gene, which encodes a mitochondrial coiled-coil domain-containing protein. The polyclonal format retains genetic heterogeneity, allowing robust assessment of gene function without clonal selection bias, and is optimized for in vitro assays probing mitochondrial roles in gastric cancer.
AGS is an epithelial cell line established from a gastric adenocarcinoma and serves as a prevalent model for studying gastric carcinogenesis. Its adherent growth and well-characterized genomic landscape facilitate gene editing and downstream phenotypic analyses. The CCDC90B knockout derivatives preserve the malignant features of the parental line, enabling direct comparison between wild-type and gene-disrupted populations to discern CCDC90B-specific contributions to tumor cell behavior.
CCDC90B localizes to mitochondria and contains a coiled-coil domain suggestive of protein?Cprotein interaction functions. Though its regulatory network remains poorly defined, CCDC90B is implicated in mitochondrial homeostasis, with mechanistic links to respiratory chain complexes, BCL-2 family members (e.g., BAX, BAK), cytochrome c release, and caspase activation. Disruption of CCDC90B is therefore predicted to impair mitochondrial integrity, potentially shifting the balance of pro- and anti-apoptotic signals and altering cellular energy metabolism.
In AGS gastric cancer cells, which often depend on robust mitochondrial activity for proliferation and survival, CCDC90B knockout provides a defined model to study metabolic vulnerability and apoptotic sensitivity. The polyclonal design mitigates clonal artifacts, ensuring that phenotypes??such as reduced metabolic flux, decreased colony formation, or enhanced apoptosis under stress??reflect authentic gene-dependent effects. This makes the model particularly valuable for target validation studies in gastric oncology.
This product supports a broad array of experimental techniques, including western blotting and RT-qPCR for expression profiling, MTT and colony formation assays for growth assessment, JC-1 and Seahorse analyses for mitochondrial membrane potential and bioenergetics, and Annexin V staining for apoptosis detection. These tools enable comprehensive dissection of CCDC90B??s role in mitochondrial function and cell death pathways. For further information, please contact Ascent Research.
