The CCDC90B Knockout HAP1 Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population derived from the HAP1 cell line, designed for functional interrogation of the CCDC90B gene. This product provides a research tool for studying the poorly characterized coiled-coil domain-containing protein encoded by CCDC90B. The polyclonal knockout format offers a mixed population of edited cells, facilitating robust phenotypic analysis without clonal bias. This model is intended to support functional genomics, phenotypic screening, and elucidation of the gene??s cellular role.
HAP1 is a near-haploid cell line derived from the KBM-7 chronic myeloid leukemia (CML) line, widely used as a model system for hematopoietic cell biology and genetic screening. The near-haploid karyotype simplifies gene editing and reduces genetic redundancy, enabling effective loss-of-function studies. HAP1 cells exhibit adherent growth and retain key signaling pathways relevant to CML biology, making them a versatile substrate for knockout investigations. The haploid state facilitates efficient disruption of target genes in pooled populations, supporting polyclonal knockout approaches.
CCDC90B encodes a coiled-coil domain-containing protein whose molecular function remains uncharacterized. No upstream regulators, downstream targets, or interacting partners have been reported for this protein, and its participation in specific signaling pathways is currently unknown. The coiled-coil domain suggests potential involvement in protein?Cprotein interactions, but direct experimental evidence is lacking. The CRISPR-mediated loss-of-function model provided by this product enables systematic dissection of its roles in cellular processes, including potential effects on proliferation, survival, or differentiation, thereby generating hypotheses for its biological significance and identifying functional interactors.
In the context of the HAP1 host cell, the CCDC90B knockout polyclonal cells offer a clean system for exploring gene function in a hematopoietic environment. HAP1 cells are particularly suited for genetic screening due to their haploid genome, which reduces the masking effect of a second allele. The polyclonal format enables high-throughput phenotypic assays to detect changes in cell fitness, morphology, or signaling in response to gene disruption. Consequently, this model can be integrated into arrayed screens or pooled CRISPR screening strategies to uncover CCDC90B-dependent phenotypes in CML-derived cells.
Researchers can apply this polyclonal knockout model in a range of functional assays, including Western blotting and RT-qPCR to confirm target disruption, immunofluorescence to assess protein localization or cellular morphology, and proliferation, cell cycle, and apoptosis assays to evaluate growth and survival impacts. These approaches address key questions in functional genomics and phenotypic screening, contributing to the elucidation of uncharacterized genes. For additional product support or technical consultation, please contact Ascent Research.
